AB69479

Anti-VEGF 165A antibody [6B7]

Name: Anti-VEGF 165A antibody [6B7] (ab69479) | Abcam
Brand: Abcam Limited
SKU: AB69479
Price: 430 USD
Availability: InStock

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(55 Publications)

Mouse Monoclonal VEGFA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 55 publications. Immunogen corresponding to Full Length Protein corresponding to Human VEGFA.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

2 Images

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Immunoprecipitation - Anti-VEGF 165A antibody [6B7] (AB69479)

Lane 1- 1µg VEGF immunoprecipitated with 2µg antibody. Lane 2- HUVEC lysate immunoprecipitated with 2µg antibody.

All lanes:

Immunoprecipitation - Anti-VEGF 165A antibody [6B7] (ab69479)

Predicted band size: 27 kDa

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Western blot - Anti-VEGF 165A antibody [6B7] (AB69479)

All lanes:

Western blot - Anti-VEGF 165A antibody [6B7] (ab69479)

All lanes:

VEGF at 0.1 µg

Predicted band size: 27 kDa

Observed band size: 23 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6B7

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Full Length Protein corresponding to Human VEGFA. The exact immunogen used to generate this antibody is proprietary information.

P15692

Specificity

This antibody reacts with native and denatured human and mouse VEGF. The immunogen is Human Vascular Endothelial Growth Factor 165A, but this has high homology with multiple VEGF isoforms, therefore it should recognise these other isoforms too.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF 165A or Vascular Endothelial Growth Factor 165A is an important protein that plays a role in angiogenesis which is the growth of new blood vessels. Also known as VEGF-A or VEGF isoform 165 this protein has a mass of around 23 kDa when it is in its biotinylated form. VEGF 165A is expressed in various cell types including endothelial cells which form the lining of blood vessels as well as in smooth muscle cells and certain tumor cells. The protein is often targeted in research using anti-VEGF antibodies.

Biological function summary

This protein acts directly on endothelial cells to stimulate their proliferation and migration. VEGF 165A is part of a larger family of VEGF proteins that share similar structures and functions. It plays a critical role in both physiological processes like wound healing and pathological conditions like cancer where its expression becomes dysregulated. The protein can bind to receptors on the cell surface initiating cell signaling pathways that lead to angiogenesis.

Pathways

VEGF 165A functions prominently in the VEGF signaling pathway and the MAPK/ERK pathway. Through these pathways it interacts with other proteins such as VEGF receptors particularly VEGFR-2 to propagate signals leading to angiogenesis and increased vascular permeability. These pathways are vital in ensuring that tissues receive an appropriate blood supply under various conditions including growth and repair processes.

VEGF 165A shows an association with cancer and age-related macular degeneration (AMD). Overexpression of VEGF 165A is linked to tumor growth because it helps form the blood vessels that supply the tumor known as tumor angiogenesis. Similarly in AMD abnormal blood vessel growth driven by VEGF 165A leads to damage in the retina. Anti-VEGF therapies often target this protein to treat these conditions by inhibiting its action on blood vessel formation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Publications (55)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:58 PubMed39819480

2025

Tetramethylpyrazine promotes osteo-angiogenesis during bone fracture repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangbo Ai,Jingzhou Zheng

IUBMB life 76:972-986 PubMed38873890

2024

Parecoxib inhibits tumorigenesis and angiogenesis in hepatocellular carcinoma through ERK-VEGF/MMPs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Tian,YuQi Huang,Yan Liu,JiangWei Liu,Yan Liu

Clinical proteomics 21:29 PubMed38594611

2024

Proteomics study of primary and recurrent adamantinomatous craniopharyngiomas.

Applications

Unspecified application

Species

Unspecified reactive species

Haidong Deng,Ting Lei,Siqi Liu,Wenzhe Hao,Mengqing Hu,Xin Xiang,Ling Ye,Dongting Chen,Yan Li,Fangjun Liu

Biomedical journal 47:100675 PubMed37944864

2023

Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Ke,Changhan Chen,Ming Yang,Hao Chen,Youhui Ke,Liqun Li

iScience 26:108236 PubMed37953957

2023

Exosomes derived from platelet-rich plasma promote diabetic wound healing via the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhai Cao,Xiaotong Meng,Fangming Cao,Jinpeng Wang,Maowei Yang

iScience 26:106804 PubMed37235058

2023

Hypoxia-induced loss of SRSF2-dependent DNA methylation promotes CTCF-mediated alternative splicing of VEGFA in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Yadav,Anchala Pandey,Parik Kakani,Srinivas Abhishek Mutnuru,Atul Samaiya,Jharna Mishra,Sanjeev Shukla

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 261:1321-1329 PubMed36445446

2022

The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Rossella Colabelli Gisoldi,Gemma Lodato,Bijorn Omar Balzamino,Graziana Esposito,Alessandra Micera,Augusto Pocobelli

European journal of histochemistry : EJH 66: PubMed36305269

2022

VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-κB/ICAM-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Zhang,Min Zhou,Xia Cai,Yan Zhou,Xueling Jiang,Yan Luo,Yue Hu,Rong Qiu,Yanrong Wu,Yuejin Zhang,Yan Xiong

Frontiers in bioengineering and biotechnology 10:976706 PubMed36213074

2022

Treatment outcomes of injectable thermosensitive hydrogel containing bevacizumab in intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Juehan Wang,Qinghong Xia,Lei Wu,Fei Chen,Li Li,Ce Zhu,Miaomiao He,Yulin Jiang,Yong Huang,Hong Ding,Ruibang Wu,Li Zhang,Yueming Song,Liming Liu

Molecular medicine reports 25: PubMed35088884

2022

bark extract rich in flavonoids promotes hair growth in dorsal skin by regulating inflammatory cytokines and increasing growth factors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Young Her,Tae-Kyeong Lee,Hyejin Sim,Jae-Chul Lee,Dae Won Kim,Soo Young Choi,Jun Kee Hong,Ji-Won Lee,Jong-Dai Kim,Moo-Ho Won,Sung-Su Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGF 165A antibody [6B7]

Immunoprecipitation - Anti-VEGF 165A antibody [6B7] (AB69479)

Western blot - Anti-VEGF 165A antibody [6B7] (AB69479)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (55)

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