AB106580

Anti-VEGF 165A antibody

Name: Anti-VEGF 165A antibody (ab106580) | Abcam
Brand: Abcam Limited
SKU: AB106580
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal VEGFA antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VEGFA.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images

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Western blot - Anti-VEGF 165A antibody (AB106580)

All lanes:

Western blot - Anti-VEGF 165A antibody (ab106580) at 1/1000 dilution

All lanes:

Recombinant human VEGF 165A

Secondary

All lanes:

Peroxidase conjugated anti-Rabbit IgG at 1/40000 dilution

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VEGFA.

P15692

Specificity

In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. This purified antibody has been heated to 56°C for 30 minutes.

Storage buffer

Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF 165A or Vascular Endothelial Growth Factor 165A is an important protein that plays a role in angiogenesis which is the growth of new blood vessels. Also known as VEGF-A or VEGF isoform 165 this protein has a mass of around 23 kDa when it is in its biotinylated form. VEGF 165A is expressed in various cell types including endothelial cells which form the lining of blood vessels as well as in smooth muscle cells and certain tumor cells. The protein is often targeted in research using anti-VEGF antibodies.

Biological function summary

This protein acts directly on endothelial cells to stimulate their proliferation and migration. VEGF 165A is part of a larger family of VEGF proteins that share similar structures and functions. It plays a critical role in both physiological processes like wound healing and pathological conditions like cancer where its expression becomes dysregulated. The protein can bind to receptors on the cell surface initiating cell signaling pathways that lead to angiogenesis.

Pathways

VEGF 165A functions prominently in the VEGF signaling pathway and the MAPK/ERK pathway. Through these pathways it interacts with other proteins such as VEGF receptors particularly VEGFR-2 to propagate signals leading to angiogenesis and increased vascular permeability. These pathways are vital in ensuring that tissues receive an appropriate blood supply under various conditions including growth and repair processes.

VEGF 165A shows an association with cancer and age-related macular degeneration (AMD). Overexpression of VEGF 165A is linked to tumor growth because it helps form the blood vessels that supply the tumor known as tumor angiogenesis. Similarly in AMD abnormal blood vessel growth driven by VEGF 165A leads to damage in the retina. Anti-VEGF therapies often target this protein to treat these conditions by inhibiting its action on blood vessel formation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:494 PubMed39695175

2024

MACC1 ablation suppresses the dedifferentiation process of non-CSCs in lung cancer through stabilizing KLF4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoshi Li,Shiqing Wang,Tao Guo,Xinyi Yan,Chaoqun Chen,Wenjing Zhang,Jinyao Zhao,Jinrui Zhang,Shilei Zhao,Yang Wang,Yangfan Qi,Chundong Gu

Materials today. Bio 19:100590 PubMed36910272

2023

A novel implant surface modification mode of Fe3O4-containing TiO2 nanorods with sinusoidal electromagnetic field for osteoblastogenesis and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ranyue Ren,Jiachao Guo,Hao Song,Yong Wei,Chao Luo,Yayun Zhang,Liangxi Chen,Biao Gao,Jijiang Fu,Wei Xiong

The journal of gene medicine 23:e3369 PubMed34057770

2021

VEGF gene-modified human umbilical cord blood mesenchymal stem cells protect against acute liver failure in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Haiou Chen,Shigang Tang,Jinmao Liao,Meng Liu,Yihe Lin

Oncology reports 44:722-734 PubMed32468055

2020

Long non‑coding RNA MIAT knockdown potentiates the therapeutic effect of transcatheter arterial embolization in liver cancer by regulating the miR‑203a/HIF‑1α axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Liu,Guangshao Cao,Jianwen Liu,Xiaoyang Zhao,Huicun Cao

Ophthalmic research 49:90-9 PubMed23257772

2012

Modeling of diseases of retinal ischemia in vitro: possible participation of autocrine vascular endothelial growth factor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yan,Tao He,Yin Shen,Xiao Chen,Bo Diao,Zhi Li,Fang Zhou,Yi-Qiao Xing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGF 165A antibody

Western blot - Anti-VEGF 165A antibody (AB106580)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise