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AB291246

Anti-VEGF 165A antibody - BSA and Azide free

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Goat Polyclonal VEGFA antibody. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VEGFA aa 1 to C-terminus.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

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Sandwich ELISA - Anti-VEGF 165A antibody - BSA and Azide free (AB291246)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-VEGF 165A antibody - BSA and Azide free (AB291246)

To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab291246 is required. This antibody, in conjunction with ab271201 as a biotinylated detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.

Western blot - Anti-VEGF 165A antibody - BSA and Azide free (AB291246)
  • WB

Supplier Data

Western blot - Anti-VEGF 165A antibody - BSA and Azide free (AB291246)

To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane.

Gel run under non-reducing conditions.

Lane 1 : Marker

Lanes 2-12 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human VEGF165, respectively.

All lanes:

Western blot - Anti-VEGF 165A antibody - BSA and Azide free (ab291246)

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human VEGFA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15692

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Reactivity data

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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration of 0.1-1.0mg/ml.
Purification technique
Affinity purification Immunogen
Purification notes
Purified by affinity chromatography employing immobilized hVEGF matrix.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF 165A or Vascular Endothelial Growth Factor 165A is an important protein that plays a role in angiogenesis which is the growth of new blood vessels. Also known as VEGF-A or VEGF isoform 165 this protein has a mass of around 23 kDa when it is in its biotinylated form. VEGF 165A is expressed in various cell types including endothelial cells which form the lining of blood vessels as well as in smooth muscle cells and certain tumor cells. The protein is often targeted in research using anti-VEGF antibodies.
Biological function summary

This protein acts directly on endothelial cells to stimulate their proliferation and migration. VEGF 165A is part of a larger family of VEGF proteins that share similar structures and functions. It plays a critical role in both physiological processes like wound healing and pathological conditions like cancer where its expression becomes dysregulated. The protein can bind to receptors on the cell surface initiating cell signaling pathways that lead to angiogenesis.

Pathways

VEGF 165A functions prominently in the VEGF signaling pathway and the MAPK/ERK pathway. Through these pathways it interacts with other proteins such as VEGF receptors particularly VEGFR-2 to propagate signals leading to angiogenesis and increased vascular permeability. These pathways are vital in ensuring that tissues receive an appropriate blood supply under various conditions including growth and repair processes.

VEGF 165A shows an association with cancer and age-related macular degeneration (AMD). Overexpression of VEGF 165A is linked to tumor growth because it helps form the blood vessels that supply the tumor known as tumor angiogenesis. Similarly in AMD abnormal blood vessel growth driven by VEGF 165A leads to damage in the retina. Anti-VEGF therapies often target this protein to treat these conditions by inhibiting its action on blood vessel formation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
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Product promise

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