Anti-VEGF Receptor 1 antibody [EWF]

• IP

Collaborator

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

Immunoprecipitation of VEGFR1 in A-375 cells. Lysates were prepared and immunoprecipitation was performed using 2 μg of ab11934 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

false

• WB

Collaborator

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

ab11934 was shown to react with VEGFR1 in wild-type A-375 cells in Western blot with loss of signal observed in a VEGFR1 knockout cell line. Wild-type A-375 and VEGFR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab11934 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 1/500 dilution

Lane 1:

Wild-type A-375 lysate at 20 µg

Lane 2:

VEGFR1 knock-out A-375 lysate at 20 µg

false

• WB

Supplier Data

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

Lane 1:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 10 mg/mL

Lane 2:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 10 µg/mL

Lane 1:

Recombinant human soluble VEGF Receptor 1

Lane 2:

Supernantant from Adenovirus Infected A549 cells expressing mouse VEGF Receptor 1

Predicted band size: 151 kDa

false

• WB

Supplier Data

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 10 µg/mL

All lanes:

Conditioned media from insect cells expressing recombinant human soluble VEGF Receptor 1

Predicted band size: 151 kDa

false

• WB

Supplier Data

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (AB11934)

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [EWF] (ab11934) at 10 µg/mL

All lanes:

Conditioned media from insect cells expressing recombinant human soluble VEGF Receptor 1

Predicted band size: 151 kDa

false