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Rabbit Recombinant Monoclonal VEGF Receptor 1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat, Chinese hamster samples. Cited in 307 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Expected
Not recommended
Tested
Not recommended
Mouse
Expected
Tested
Not recommended
Tested
Not recommended
Rat
Expected
Predicted
Not recommended
Predicted
Not recommended
Chinese hamster
Predicted
Predicted
Not recommended
Expected
Not recommended

Tested
Tested

Species

Human

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Chinese hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30.00000 - 1/100.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chinese hamster, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Chinese hamster

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000.00000 - 1/5000.00000

Notes

-

Species

Human

Dilution info

1/1000.00000 - 1/5000.00000

Notes

-

Expected
Expected

Species

Chinese hamster

Dilution info

1/1000.00000 - 1/5000.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Chinese hamster, Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (Ref.11). May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1 (PubMed:16685275).Isoform 1Phosphorylates PLCG.Isoform 2May function as decoy receptor for VEGFA.Isoform 3May function as decoy receptor for VEGFA.Isoform 4May function as decoy receptor for VEGFA.Isoform 7Has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.

Alternative names

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Rabbit Recombinant Monoclonal VEGF Receptor 1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat, Chinese hamster samples. Cited in 307 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

Y103

Purification technique

Affinity purification Protein A

Specificity

Based on the antibody's immunogen sequence, it recognises 151 kDa VEGF receptor 1/Flt1, splice isoforms sFlt1 (77 kDa) and sFlt1-14 (82 kDa), and isoform 4 (61 kDa). The sequence is not present in isoforms 5-8 based on Uniprot ID P17948.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152), expandable thumbnail
    Image from Sano M et al. PLoS ONE. 2014 Mar 20; 9(3). Fig 2E. DOI 10.1371/journal.pone.0089830. Lymphangiogenesis and Angiogenesis in Abdominal Aortic Aneurysm. e89830.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.

    Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 μm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H2O2 in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

  • Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (ab32152), expandable thumbnail

    Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    VEGF Receptor 1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab32152 at 1:30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab32152 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.

    Lane 1: Mouse brain lysate 10μg.

    Lane 2: ab32152 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32152 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (AB32152)

    Predicted band size: 151 kDa

  • Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152), expandable thumbnail

    Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VEGF Receptor 1 antibody [Y103] (AB32152) at 1/1000 dilution

    Lane 1: Mouse brain lysates at 15 µg

    Lane 2: Human brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 151 kDa

    Observed band size: 180 kDa

    Exposure time: 40s

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab32152).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling VEGF with ab32152, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human gastric carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152), expandable thumbnail

    Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    All lanes: Western blot - Anti-VEGF Receptor 1 antibody [Y103] (AB32152) at 1/10000 dilution

    All lanes: mouse brain tissue

    Predicted band size: 151 kDa

    Observed band size: 180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed, 1% TritonX-100 in PBS permeabilized human Metastatic melanoma tissue. Stained with ab32152 at 1/100 dilution. Secondary antibody used was HRP Donkey Jackson Immuno Anti-rabbit at 1/500 dilution. Blocking was done with 3% donkey serum for 30 minutes at 25°C. The sample was incubated with the primary antibody and 1% donkey serum for 1 hour at 25°C. Antigen retrieval method was heat mediated Tris-EDTA

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Product protocols

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