Anti-VEGF Receptor 1 antibody [Y103]

6 product images

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  Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  VEGF Receptor 1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab32152 at 1:30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab32152 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:1000 dilution.
  

  Lane 1: Mouse brain lysate 10μg.

  Lane 2: ab32152 IP in mouse brain lysate.

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32152 in mouse brain lysate.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 1 second.

  All lanes: Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  Predicted band size: 151 kDa

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  Image from Sano M et al. PLoS ONE. 2014 Mar 20; 9(3). Fig 2E. DOI 10.1371/journal.pone.0089830. Lymphangiogenesis and Angiogenesis in Abdominal Aortic Aneurysm. e89830.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.
  

  Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 μm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H₂O₂ in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

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  Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  All lanes: Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/1000 dilution

  Lane 1: Mouse brain lysates at 15 µg

  Lane 2: Human brain lysates at 15 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 151 kDa

  Observed band size: 180 kDa

  Exposure time: 40s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling VEGF with ab32152, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human gastric carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

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  Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  All lanes: Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution

  All lanes: mouse brain tissue

  Predicted band size: 151 kDa

  Observed band size: 180 kDa

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed, 1% TritonX-100 in PBS permeabilized human Metastatic melanoma tissue. Stained with ab32152 at 1/100 dilution. Secondary antibody used was HRP Donkey Jackson Immuno Anti-rabbit at 1/500 dilution. Blocking was done with 3% donkey serum for 30 minutes at 25°C. The sample was incubated with the primary antibody and 1% donkey serum for 1 hour at 25°C. Antigen retrieval method was heat mediated Tris-EDTA