Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (AB210608)

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling VEGF with ab32152, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human gastric carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab32152).

• IP

Lab

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (AB210608)

VEGF Receptor 1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab32152 at 1 : 30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab32152 1 : 1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.

Lane 1 : Mouse brain lysate 10μg.

Lane 2 : ab32152 IP in mouse brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab32152 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab32152).

All lanes:

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (<a href='/en-us/products/primary-antibodies/vegf-receptor-1-antibody-y103-ab32152'>ab32152</a>)

Predicted band size: 151 kDa

false

• WB

Lab

Western blot - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (AB210608)

This data was developed using ab32152, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysates were not boiled.

Lanes 1 - 2:

Western blot - Anti-VEGF Receptor 1 antibody [Y103] (<a href='/en-us/products/primary-antibodies/vegf-receptor-1-antibody-y103-ab32152'>ab32152</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)

Lanes 1 - 2:

Western blot - Anti-VEGF Receptor 1 antibody [Y103] - Low endotoxin, Azide free (<a href='/en-us/products/primary-antibodies/vegf-receptor-1-antibody-y103-low-endotoxin-azide-free-ab184784'>ab184784</a>)

Lanes 1 - 2:

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-epr20547-chip-grade-ab213204'>ab213204</a>) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell) cells transfected with sFlt1 (P17948-2) expression vector containing a His -tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 77 kDa

false

Exposure time: 5s

• WB

Lab

Western blot - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (AB210608)

This data was developed using ab32152, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) 1 : 5,000 dilution used as loading control.

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [Y103] (<a href='/en-us/products/primary-antibodies/vegf-receptor-1-antibody-y103-ab32152'>ab32152</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector contain a His-tag whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human VEGF Receptor 1 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (AB210608)

This data was developed using ab32152, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

All lanes:

Western blot - Anti-VEGF Receptor 1 antibody [Y103] (<a href='/en-us/products/primary-antibodies/vegf-receptor-1-antibody-y103-ab32152'>ab32152</a>) at 1/1000 dilution

Lane 1:

Mouse placenta tissue lysate at 20 µg

Lane 2:

Rat placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa

false

Exposure time: 10s