Rabbit Polyclonal VEGF Receptor 2 antibody. Suitable for IHC-P, IP, Flow Cyt, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat, Dog, Cow samples. Cited in 139 publications. Immunogen corresponding to Synthetic Peptide within Mouse Kdr.
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- Molecular medicine (Cambridge, Mass.) 29:1552023Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui Hu,Changwei Lin,Kai Gao,Miao Chen,Fei Long,Buning TianPubMed 37940881
- Nanoscale 15:17173-171832023Effects of graphene oxide and reduced graphene oxide nanomaterials on porcine endothelial progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Polo-Montalvo,Mónica Cicuéndez,Laura Casarrubios,Nathalie Barroca,Daniela da Silva,María José Feito,Rosalía Diez-Orejas,María Concepción Serrano,Paula A A P Marques,María Teresa PortolésPubMed 37853851
- Nature communications 14:48932023Camrelizumab combined with apatinib in patients with first-line platinum-resistant or PD-1 inhibitor resistant recurrent/metastatic nasopharyngeal carcinoma: a single-arm, phase 2 trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Yuan,Guo-Dong Jia,Xiao-Fei Lv,Si-Yi Xie,Shan-Shan Guo,Da-Feng Lin,Li-Ting Liu,Dong-Hua Luo,Yi-Fu Li,Shen-Wen Deng,Ling Guo,Mu-Sheng Zeng,Xiu-Yu Cai,Sai-Lan Liu,Xue-Song Sun,Xiao-Yun Li,Su-Chen Li,Qiu-Yan Chen,Lin-Quan Tang,Hai-Qiang MaiPubMed 37580352
- Bioengineering & translational medicine 8:e105622023Dual gene-activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of VEGF and angiopoietin-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Weng,Min Yang,Wei Zhang,Ronghua Jin,Sizhan Xia,Manjia Zhang,Pan Wu,Xiaojie He,Chunmao Han,Xiong Zhao,Xingang WangPubMed 37693053
- Investigational new drugs 41:226-2392023Development of a MET-targeted single-chain antibody fragment as an anti-oncogene targeted therapy for breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRana Vafaei,Zohreh Khaki,Malihe Salehi,Neda Jalili,Mohammad Reza Esmailinejad,Ahad Muhammadnajad,Seyed Mahdi Nassiri,Alireza Vajhi,Shima Moradi Kalbolandi,Roya Mirzaei,Leila FarahmandPubMed 37004643
- The Tohoku journal of experimental medicine 260:63-732023Rhamnazin Enhanced Anti-Tumor Efficacy of Anti-PD-1 Therapy for Lung Cancer in Mice through Inhibition of PD-L1 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Shi Wang,Ye Liu,Xuan Ting Zhang,Dong Qiang YuPubMed 36823182
- Scientific reports 13:30002023Investigation of SAMD1 ablation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruce Campbell,Lisa M Weber,Sandra J Engle,Terence R S Ozolinš,Patricia Bourassa,Robert Aiello,Robert LiefkePubMed 36810619
- Cell proliferation 56:e133662022CD34 cells identified as pluripotent stem cell-derived definitive hemogenic endothelium purified using bone morphogenetic protein 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoo-Been Jeon,A-Reum Han,Sunghun Lee,Seung Chan Lee,Min Ji Lee,Soon-Jung Park,Sung-Hwan Moon,Ji Yoon LeePubMed 36478274
- American journal of translational research 14:6712-67252022Transplantation of bFGF-transfected bone mesenchymal stem cells on collagen scaffolds promotes the regeneration of injured rat endometrium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Chen,Yingxin Gong,Ninghong Jiang,Jingjing Xiao,Yaping Wang,Limei Chen,Long SuiPubMed 36247308
- Cancer science 113:3148-31602022Vascular endothelial growth factor receptor 2 expression and immunotherapy efficacy in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenji Nakahama,Hiroyasu Kaneda,Masahiko Osawa,Mitsuru Fukui,Motohiro Izumi,Naoki Yoshimoto,Akira Sugimoto,Hiroaki Nagamine,Koichi Ogawa,Yoshiya Matsumoto,Kenji Sawa,Yoko Tani,Shigeki Mitsuoka,Tetsuya Watanabe,Kazuhisa Asai,Tomoya KawaguchiPubMed 35722982
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Kdr. The exact immunogen used to generate this antibody is proprietary information. Database link P35918
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Expected |
| Mouse | Predicted | Expected | Expected | Expected | Expected |
| Rat | Predicted | Expected | Expected | Expected | Expected |
| Cow | Predicted | Expected | Expected | Expected | Expected |
| Dog | Predicted | Expected | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Mouse, Cow, Rat | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Dog | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes See Abreview. |
Species Rat | Dilution info - | Notes See Abreview. |
Species Human | Dilution info - | Notes See Abreview. |
Species Dog | Dilution info - | Notes See Abreview. |
Species Cow | Dilution info - | Notes See Abreview. |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1
Recommended products
Rabbit Polyclonal VEGF Receptor 2 antibody. Suitable for IHC-P, IP, Flow Cyt, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat, Dog, Cow samples. Cited in 139 publications. Immunogen corresponding to Synthetic Peptide within Mouse Kdr.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Kdr. The exact immunogen used to generate this antibody is proprietary information. Database link P35918
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Isoform 1 localization: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.
Note: Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
- Biological function summary
VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.
- Pathways
VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.
- Associated diseases and disorders
VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Image from Arias-Pulido, Hugo et al., BMC Cancer 12 (2012): 298. PMC. Web. 25 Jan. 2017. doi: 10.1186/1471-2407-12-298. Fig 1I. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0/.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.
Image from Arias-Pulido, Hugo et al., BMC Cancer 12 (2012): 298. PMC. Web. 25 Jan. 2017. doi: 10.1186/1471-2407-12-298. Fig 1H. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
Formalin fixed, paraffin-embedded human angiosarcoma tissue stained for VEGF Receptor 2 using ab2349 at 1/50 dilution in immunohistochemical analysis.
This image is courtesy of a customer review submitted by Manoj Kumar ValluruImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
ab2349 staining the VEGF Receptor 2 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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