Rabbit Polyclonal VEGF Receptor 2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 27 publications.
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- Cancers 15:2023miR-146a-5p Promotes Angiogenesis and Confers Trastuzumab Resistance in HER2+ Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaula Cabello,Sandra Torres-Ruiz,Anna Adam-Artigues,Jaume Forés-Martos,María Teresa Martínez,Cristina Hernando,Sandra Zazo,Juan Madoz-Gúrpide,Ana Rovira,Octavio Burgués,Federico Rojo,Joan Albanell,Ana Lluch,Begoña Bermejo,Juan Miguel Cejalvo,Pilar ErolesPubMed 37046799
- Annals of translational medicine 11:1972023Inhibition of NF-κB ameliorates aberrant retinal glia activation and inflammatory responses in streptozotocin-induced diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyi Ding,Zhongcui Sun,Yue Guo,Wenyi Tang,Qinmeng Shu,Gezhi XuPubMed 37007562
- International journal of molecular sciences 24:2023High VEGFR3 Expression Reduces Doxorubicin Efficacy in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Torres-Ruiz,Eduardo Tormo,Iris Garrido-Cano,Ana Lameirinhas,Federico Rojo,Juan Madoz-Gúrpide,Octavio Burgués,Cristina Hernando,Begoña Bermejo,María Teresa Martínez,Ana Lluch,Juan Miguel Cejalvo,Pilar ErolesPubMed 36835014
- Medical molecular morphology 56:128-1372023Gemcitabine alters sialic acid binding of the glycocalyx and induces inflammatory cytokine production in cultured endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariko Gunji,Chika Sawa,Minako Akiyama,Shumpei Mukai,Takashi Takaki,Dedong Kang,Kazuho HondaPubMed 36622466
- Heliyon 8:e124212022Hypoxic preconditioning promotes galvanotaxis of human dermal microvascular endothelial cells through NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZe Zhang,Chao Wu,Jinrui Yang,Jie Liu, Yi Li,Luojia Liu,Meng Kong,Jiaping Zhang,Xupin JiangPubMed 36643317
- Frontiers in pharmacology 13:10441152022Curcumol repressed cell proliferation and angiogenesis SP1/mir-125b-5p/VEGFA axis in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangju Ma,Xiaojuan Tang,Qing Tang,Shiyan Wang,Junhong Zhang,Yue Lu,Jingjing Wu,Ling HanPubMed 36467048
- Journal of materials science. Materials in medicine 33:122022Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Liu,Yunen Liu,Mi Wu,Rufei Zou,Shun Mao,Peifang Cong,Mingxiao Hou,Hongxu Jin,Yan Zhao,Yongli BaoPubMed 35050422
- Frontiers in molecular biosciences 8:6524432021Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Luo,Xiaobing Yang,Spyridon P Basourakos,Xuemei Zuo,Dechao Wei,Jiahui Zhao,Mingchuan Li,Qiankun Li,Tao Feng,Pengju Guo,Yongguang JiangPubMed 34746227
- Journal of orthopaedic surgery and research 16:3792021Expression of VEGF-A Signaling Pathway in Cartilage of ACLT-induced Osteoarthritis Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Jia Qian,Qi Xu,Wei-Min Xu,Ren Cai,Gui-Cheng HuangPubMed 34127028
- International journal of molecular sciences 22:2021Disabling VEGF-Response of Purkinje Cells by Downregulation of via miRNA-204-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian Gehmeyr,Abdelouahid Maghnouj,Jonas Tjaden,Matthias Vorgerd,Stephan Hahn,Veronika Matschke,Verena Theis,Carsten TheissPubMed 33671638
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P35968
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1
Recommended products
Rabbit Polyclonal VEGF Receptor 2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 27 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P35968
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
- Biological function summary
VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.
- Pathways
VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.
- Associated diseases and disorders
VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
Western blot analysis of extracts from SK-OV3 cells using VEGF Receptor 2 antibody ab39638 (Lane 1 and 2) and VEGF Receptor 2 (phospho-Tyr951) antibody (Lane 3 and 4).
Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)
All lanes: Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
Predicted band size: 151 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
Immunohistochemical analysis of human brain tissue stained with ab39638. The image on the right is blocked with the synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
Immunohistochemical analysis of human stomach cancer tissue stained with ab39638 at 1/200 dilution at 4oC overnight. The negative control was secondary antibody only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab39638)
Immunohistochemical analysis of rat spinal cord tissue stained with ab39638 at 1/200 dilution at 4oC overnight. The negative control was secondary antibody only.
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (ab39638)
ICC/IF image of ab39638 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39638, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
All lanes: Western blot - Anti-VEGF Receptor 2 antibody (ab39638)
All lanes: HeLa cell lysate
Predicted band size: 151 kDa
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