AB39638

Anti-VEGF Receptor 2 antibody

Name: Anti-VEGF Receptor 2 antibody. Rabbit polyclonal (ab39638) | Abcam
Brand: Abcam Limited
SKU: AB39638
Rating: 5 (3 reviews)

(3 Reviews)

(36 Publications)

Rabbit Polyclonal VEGF Receptor 2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 36 publications.

View Alternative Names

CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (AB39638)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (AB39638)

ICC/IF image of ab39638 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39638, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemical analysis of human brain tissue stained with ab39638. The image on the right is blocked with the synthesized peptide.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemical analysis of human stomach cancer tissue stained with ab39638 at 1/200 dilution at 4^oC overnight. The negative control was secondary antibody only.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemical analysis of rat spinal cord tissue stained with ab39638 at 1/200 dilution at 4^oC overnight. The negative control was secondary antibody only.

Unknown

Western blot - Anti-VEGF Receptor 2 antibody (AB39638)

Western blot analysis of extracts from SK-OV3 cells using VEGF Receptor 2 antibody ab39638 (Lane 1 and 2) and VEGF Receptor 2 (phospho-Tyr951) antibody (Lane 3 and 4).

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

Predicted band size: 151 kDa

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Supplier Data

Western blot - Anti-VEGF Receptor 2 antibody (AB39638)

All lanes:

Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

All lanes:

HeLa cell lysate

Predicted band size: 151 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.

Biological function summary

VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.

Pathways

VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.

VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.

See full target information KDR

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:701 PubMed41053006

2025

Lactate-mediated histone lactylation promotes melanoma angiogenesis via IL-33/ST2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Zhao,Yuxuan Qian,Lin He,Taoxin Peng,Hanbin Wang,Xiangxu Wang,Linhan Jiang,Jinrong Fan,Hengxiang Zhang,Di Qu,Qing Zhu,Hao Wang,Shida Zhang,Chenyang Li,Xiwen Dong,Xianya Zhao,Huina Wang,Yuqi Yang,Xiuli Yi,Tao Zhao,Yu Liu,Jianglin Zhang,Guoqiang Zhang,Qiong Shi,Tianwen Gao,Chunying Li,Weinan Guo

Frontiers in bioengineering and biotechnology 13:1606230 PubMed40843448

2025

Macula densa cell angiogenic mechanisms and their therapeutic potential in kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Georgina Gyarmati,Dorinne Desposito,Anne Riquier-Brison,Alejandra Becerra Calderon,Greta Trogen,Audrey Izuhara,Michifumi Yamashita,János Peti-Peterdi

Clinical epigenetics 17:82 PubMed40380281

2025

DNA hypermethylation of PLTP mediated by DNMT3B aggravates vascular dysfunction in diabetic retinopathy via the AKT/GSK3β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Cai,Chufeng Gu,Chunren Meng,Yujie Wang,Qingquan Wei,Shuai He,Dongwei Lai,Xingyun Wang,Tengfei Wang,Qinghua Qiu

International endodontic journal 58:991-1005 PubMed40106315

2025

Activin a regulates vascular formation and stabilization in direct coculture of dental pulp stem cells and endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Zhong,Yuchen Zhang,Shulan Lin,Jun Kang,Mingxin Hu,Junqing Liu,Ying Chen,Qianzhou Jiang,Chengfei Zhang

Cell death & disease 15:447 PubMed38918360

2024

IGFBP2/ITGA5 promotes gefitinib resistance via activating STAT3/CXCL1 axis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hengxiao Lu,Jiangshan Ai,Yingying Zheng,Wolong Zhou,Liming Zhang,Jiebo Zhu,Heng Zhang,Shaoqiang Wang

Journal of nanobiotechnology 22:290 PubMed38802884

2024

A SU6668 pure nanoparticle-based eyedrops: toward its high drug Accumulation and Long-time treatment for corneal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wu,Jinfa Ye,Minjie Zhang,Lingyu Zhang,Sijie Lin,Qingjian Li,Yanbo Liu,Yun Han,Caihong Huang,Yiming Wu,Yuhang Cheng,Shundong Cai,Lang Ke,Gang Liu,Wei Li,Chengchao Chu

Clinical Medicine Insights. Oncology 17:11795549231175715 PubMed37435016

2023

VEGF Mediates Tumor Growth and Metastasis by Affecting the Expression of E-Cadherin and N-Cadherin Promoting Epithelial to Mesenchymal Transition in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Yanyan Wang,Menglin Zhao,Xinwei Li,Huiyuan Li,Mingyue Tang,Zhijun Geng,Lugen Zuo,Xue Song,Zishu Wang,Qiang Wang,Fang Su

Cancers 15: PubMed37046799

2023

miR-146a-5p Promotes Angiogenesis and Confers Trastuzumab Resistance in HER2+ Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cabello,Sandra Torres-Ruiz,Anna Adam-Artigues,Jaume Forés-Martos,María Teresa Martínez,Cristina Hernando,Sandra Zazo,Juan Madoz-Gúrpide,Ana Rovira,Octavio Burgués,Federico Rojo,Joan Albanell,Ana Lluch,Begoña Bermejo,Juan Miguel Cejalvo,Pilar Eroles

Annals of translational medicine 11:197 PubMed37007562

2023

Inhibition of NF-κB ameliorates aberrant retinal glia activation and inflammatory responses in streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Ding,Zhongcui Sun,Yue Guo,Wenyi Tang,Qinmeng Shu,Gezhi Xu

International journal of molecular sciences 24: PubMed36835014

2023

High VEGFR3 Expression Reduces Doxorubicin Efficacy in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Torres-Ruiz,Eduardo Tormo,Iris Garrido-Cano,Ana Lameirinhas,Federico Rojo,Juan Madoz-Gúrpide,Octavio Burgués,Cristina Hernando,Begoña Bermejo,María Teresa Martínez,Ana Lluch,Juan Miguel Cejalvo,Pilar Eroles

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGF Receptor 2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Western blot - Anti-VEGF Receptor 2 antibody (AB39638)

Western blot - Anti-VEGF Receptor 2 antibody (AB39638)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

Product promise