Anti-VEGF Receptor 2 antibody [EPR21231]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21231] (AB234110)

ab234110 staining VEGFR2 in HUVEC cells. The cells were fixed with 10% paraformaldeyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab234110 at 5ugml then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).

• Flow Cyt

Lab

Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21231] (AB234110)

Overlay histogram showing HUVEC cells stained with ab234110 (red line).The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab234110, 1/10 dilution) for 30 min at 4°C. The secondary antibody (black line) used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 4°C. Unlabelled sample (blue line) was also used as a control.
Acquisition of >30,000 total events were collected.

• Flow Cyt

Lab

Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21231] (AB234110)

Overlay histogram showing Jurkat cells stained with ab234110 (red line).The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab234110, 1/10 dilution) for 30 min at 4°C. The secondary antibody (black line) used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 4°C. Unlabelled sample (blue line) was also used as a control.
Acquisition of >30,000 total events were collected.

Negative control : Jurkat cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21231] (AB234110)

Flow cytometry analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells staining with ab234110. Secondary used was Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution, and isotype control was Rabbit monoclonal IgG (ab172730) / Black. Gated on viable cell. Negative control : Jurkat

• ICC

Lab

Immunocytochemistry - Anti-VEGF Receptor 2 antibody [EPR21231] (AB234110)

Immunocytochemistry analysis of 100% methanol-fixed 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells staining with ab234110 at 1/500 dilution. Counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. The secondary used was ab150097 Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HUVEC cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue). Negative control : HeLa Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).