Anti-VEGF Receptor 2 antibody [EPR21233]

• Flow Cyt

Lab

Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21233] (AB237634)

Overlay histogram showing Jurkat cells stained with ab237634 (red line).The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237634, 1/10 dilution) for 30 min at 4°C. The secondary antibody (black line) used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 4°C. Unlabelled sample (blue line) was also used as a control.

Acquisition of >30,000 total events were collected.

Negative control : Jurkat cells.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21233] (AB237634)

ab237634 staining VEGFR2 in HUVEC cells. The cells were fixed with 10% paraformaldeyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab234110 at 0.1ugml then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).

• Flow Cyt

Lab

Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21233] (AB237634)

Overlay histogram showing HUVEC cells stained with ab237634 (red line).The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237634, 1/10 dilution) for 30 min at 4°C. The secondary antibody (black line) used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 4°C. Unlabelled sample (blue line) was also used as a control.

Acquisition of >30,000 total events were collected.

• Flow Cyt

Lab

Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21233] (AB237634)

Flow cytometry analysis of HUVEC (human umbilical vein endothelial cell) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells staining with ab237634 at 1/500 dilution. Secondary used was Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution, and isotype control was Rabbit monoclonal IgG (ab172730) / Black. The unlabelled control was cells without incubation with primary antibody and secondary antibody / Blue. Gated on viable cell. Negative control : Jurkat