Rabbit Recombinant Monoclonal VEGF Receptor 2 antibody. Carrier free. Suitable for Flow Cyt, I-ELISA, ICC/IF and reacts with Mouse, Recombinant full length protein - Mouse samples.
Images
![Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--flow-cytometry-img51188.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106)")
![Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--flow-cytometry-img13452.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106)")
![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--immunocytochemistry-immunofluorescence-img108682.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106)")
![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--immunocytochemistry-immunofluorescence-img28589.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106)")
![Indirect ELISA - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--indirect-elisa-img21462.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (AB234106)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | I-ELISA | ICC/IF | |
|---|---|---|---|
| Mouse | Tested | Expected | Tested |
| Recombinant full length protein - Mouse | Not recommended | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
3 products for Alternative Version
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
CD309, Flk-1, Flk1, Kdr, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase NYK, Protein-tyrosine kinase receptor flk-1, FLK-1
Recommended products
Rabbit Recombinant Monoclonal VEGF Receptor 2 antibody. Carrier free. Suitable for Flow Cyt, I-ELISA, ICC/IF and reacts with Mouse, Recombinant full length protein - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21884-236
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab234106 is the carrier-free version of Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
- Biological function summary
VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.
- Pathways
VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.
- Associated diseases and disorders
VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--flow-cytometry-img51188.jpg "Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)")
Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line) cell line treated with 1 μg/ml Brefeldin A for 3 hours (red) and an untreated control (green) labeling VEGF Receptor 2 with Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693 at 1/500 dilution treated with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
BFA treatment reduced cell surface VEGF Receptor 2 [PMID: 21063020].
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693).
![Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--flow-cytometry-img13452.jpg "Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)")
Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line, right) and NIH/3T3 (mouse embryo fibroblast cell line, left) cell line labeling VEGF Receptor 2 with Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693 at 1/500 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: NIH/3T3 [PMID: 20978347].
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693).
![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--immunocytochemistry-immunofluorescence-img108682.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)")
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embryo fibroblast cell line) cells labeling VEGF Receptor 2 with Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing perinuclear staining in bEnd.3 cells.
Negative control: NIH/3T3 [PMID: 20978347].
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693).
![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--immunocytochemistry-immunofluorescence-img28589.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)")
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (mouse brain endothelioma) cells labeling VEGF Receptor 2 with Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Redistribution of the VEGF Receptor 2 from the perinuclear Golgi to vesicular structures throughout the cytosol after treatment with Brefeldin A (1 μg/ml, 3 hours) in bEnd.3. BFA treatment alters the subcellular localisation of VEGF Receptor 2 [PMID: 21063020].
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693).
![Indirect ELISA - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106), expandable thumbnail](https://content.abcam.com/products/images/vegf-receptor-2-antibody-epr21884-236-bsa-and-azide-free-ab234106--indirect-elisa-img21462.jpg "Indirect ELISA - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)")
Indirect ELISA - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
This data was developed using Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693, the same antibody clone in a different buffer formulation.ELISA analysis of Mouse VEGF R2 recombinant protein at 1000 ng/ml with Anti-VEGF Receptor 2 antibody [EPR21884-236] ab233693. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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