AB134191

Anti-VEGF Receptor 2 antibody [EPRER16Y]

20ul selling size
RabMAb
Recombinant
What is this?

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(38 Publications)

Rabbit Recombinant Monoclonal VEGF Receptor 2 antibody. Suitable for IP, ELISA, WB and reacts with Recombinant fragment - Human, Human samples. Cited in 38 publications.

View Alternative Names

CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1

2 Images

ELISA - Anti-VEGF Receptor 2 antibody [EPRER16Y] (AB134191)

ELISA

Unknown

ELISA - Anti-VEGF Receptor 2 antibody [EPRER16Y] (AB134191)

ELISA analysis of Hu VEGFR2 recombinant protein at 1000 ng/mL with ab134191. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Unknown

Western blot - Anti-VEGF Receptor 2 antibody [EPRER16Y] (AB134191)

All lanes:

Western blot - Anti-VEGF Receptor 2 antibody [EPRER16Y] (ab134191) at 1/500 dilution

All lanes:

Human placenta lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 151 kDa

Observed band size: 210 kDa,230 kDa

false

Carrier free

Anti-VEGF Receptor 2 antibody [EPRER16Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPRER16Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

It is predicted that this antibody does not cross react with other members of the VEGF receptor family.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.

Biological function summary

VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.

Pathways

VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.

VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.

See full target information KDR

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:299 PubMed40935839

2025

Coinhibition of the MEK/RTK pathway has high therapeutic efficacy in KRAS-mutant non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Lu,Minjuan Hu,Yikai Zhao,Tianqing Chu,Wei Zhang,Yijia Zhou,Xinlei Cai,Jun Wu,Liang Hu,Chunlei Shi,Liwen Xiong,Aiqin Gu,Huimin Wang,Yanwei Zhang,Yuqing Lou,Runbo Zhong,Zhiqiang Gao,Hongyu Liu,Chao Zhou,Yingli Wu,Liang Zhu,Hua Zhong,Hongbin Ji,Baohui Han

Cell communication and signaling : CCS 23:108 PubMed40001144

2025

CXXC5 function blockade promotes diabetic wound healing through stimulating fibroblast and vascular endothelial cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Chen,Xiaofeng Ding,Zhouji Ma,Shuai Shao,Heyan Huang,Yumeng Huang,Beizhi Wang,Hao Zhang,Qian Tan

Histology and histopathology 40:1105-1115 PubMed39564607

2024

Paeonol regulates glycolytic metabolism by downregulating BACH1 to ameliorate stemness, angiogenesis, and EMT in SiHa cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoqin Sheng,Jing Xu,Danhong Hu,Weiwei Qian,Xiangqian Xu,Jing He

Journal of virology 98:e0112924 PubMed39287389

2024

Vascular endothelial growth factor receptor 2 as a potential host target for the inhibition of enterovirus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhao,Rui Qiao,Meng Hao,Longfa Xu,Dong Wang,Yinying Lu,Jiayan Li,Jing Wu,Yi Li,Tong Cheng,Wenhong Zhang,Jincun Zhao,Pengfei Wang

Cancers 16: PubMed39272961

2024

Fibroblast Growth Factor 2 (FGF2) Activates Vascular Endothelial Growth Factor (VEGF) Signaling in Gastrointestinal Stromal Tumors (GIST): An Autocrine Mechanism Contributing to Imatinib Mesylate (IM) Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Sergei Boichuk,Pavel Dunaev,Aigul Galembikova,Elena Valeeva

iScience 27:110080 PubMed38883819

2024

Resident vascular Sca1 progenitors differentiate into endothelial cells in vascular remodeling via miR-145-5p/ERG signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangquan Ying,Lingxia Lyu,Xiaodong Xu,Zuoshi Wen,Jianing Xue,Mengjia Chen,Zhoubin Li,Liujun Jiang,Ting Chen

American journal of physiology. Lung cellular and molecular physiology 326:L770-L785 PubMed38563994

2024

Mesenchymal stromal cell extracellular vesicles improve lung development in mechanically ventilated preterm lambs.

Applications

Unspecified application

Species

Unspecified reactive species

Kurt H Albertine,Andrew Rebentisch,Elaine Dawson,Jakob Van Boerum,Emily Major,Juraj Štipka,Hannah Foreman,David Headden,Zoë Vordos,Emily Beck,Zhengming Wang,Haixia Yang,Baifeng Yu,Mar Janna Dahl,Donald M Null,Davide Bizzotto,Chiara Veneroni,Anna Lavizzari,Raffaele L Dellacà,Eleni Delavogia,S Alex Mitsialis,Stella Kourembanas

Pharmaceutical biology 62:153-161 PubMed38347502

2024

Effects of total coumarins from on exosomal miRNA expression and angiogenesis in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Dao-Hai Cheng,Zheng-Ying Su,Ji-Hua Lv,Li Wang,Yu-Yin Deng,Li Li

Chemical biology & drug design 102:749-762 PubMed37341065

2023

Hsa-miR-503-5p regulates CTDSPL to accelerate cisplatin resistance and angiogenesis of lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Han,Yan Wang

Experimental and therapeutic medicine 25:304 PubMed37229326

2023

Role of the TPX2/NCOA5 axis in regulating proliferation, migration, invasion and angiogenesis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Wang,Fulin Zhang,Peirong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGF Receptor 2 antibody [EPRER16Y]

ELISA - Anti-VEGF Receptor 2 antibody [EPRER16Y] (AB134191)

Western blot - Anti-VEGF Receptor 2 antibody [EPRER16Y] (AB134191)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

Product promise