Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) is a rabbit polyclonal antibody that is used to detect VEGF Receptor 2 in Western Blot. Suitable for Human samples.
- Over 30 publications
- Trusted since 2003
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- Advanced healthcare materials 12:e22022102023Spatiotemporal Regulation of Injectable Heterogeneous Silk Gel Scaffolds for Accelerating Guided Vertebral Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianji Wang,Keyin Liu,Jing Wang,Geng Xiang,Xiaofan Hu,Hao Bai,Wei Lei,Tiger H Tao,Yafei FengPubMed 36465008
- International journal of molecular sciences 23:2022Neuroprotective Effects of VEGF in the Enteric Nervous System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInes Hecking,Lennart Norman Stegemann,Verena Theis,Matthias Vorgerd,Veronika Matschke,Sarah Stahlke,Carsten TheissPubMed 35743202
- Journal of oncology 2022:37445602022CircLATS2 Regulates miR-520a-3p/E2F7/p-VEGFR2 Signaling Pathway to Promote Hepatocellular Carcinoma Progression and Angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYefeng Wu,Jianmao Yuan,Zhengbin Tu,Huahua ChenPubMed 35444695
- Journal of nanobiotechnology 20:72022Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPegah Zanjanchi,S Mohsen Asghari,Hassan Mohabatkar,Mostafa Shourian,Mehdi Shafiee ArdestaniPubMed 34983556
- Science advances 7:eabg95092021Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn-Michael Arpino,Hao Yin,Emma K Prescott,Sabrina C R Staples,Zengxuan Nong,Fuyan Li,Jacqueline Chevalier,Brittany Balint,Caroline O'Neil,Rokhsana Mortuza,Stephanie Milkovich,Jason J Lee,Daniel Lorusso,Martin Sandig,Douglas W Hamilton,David W Holdsworth,Tamie L Poepping,Christopher G Ellis,J Geoffrey PickeringPubMed 34826235
- Cell reports 36:1095492021CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaolin Ma,Lingegowda S Mangala,Wen Hu,Emine Bayaktar,Akira Yokoi,Wei Hu,Sunila Pradeep,Sanghoon Lee,Paul D Piehowski,Alejandro Villar-Prados,Sherry Y Wu,Michael H McGuire,Olivia D Lara,Cristian Rodriguez-Aguayo,Christopher J LaFargue,Nicholas B Jennings,Karin D Rodland,Tao Liu,Vikas Kundra,Prahlad T Ram,Sundaram Ramakrishnan,Gabriel Lopez-Berestein,Robert L Coleman,Anil K SoodPubMed 34407412
- Cancer prevention research (Philadelphia, Pa.) 14:687-6962021Dopamine Prevents Ultraviolet B-induced Development and Progression of Premalignant Cutaneous Lesions through its D Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Lu,Madhavi Bhat,Sara Peters,Rita Mitra,Xiaokui Mo,Tatiana M Oberyszyn,Partha Sarathi Dasgupta,Sujit BasuPubMed 33846213
- Arthritis research & therapy 22:2732020Chebulinic acid is a safe and effective antiangiogenic agent in collagen-induced arthritis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Lu et. al.PubMed 33225986
- Frontiers in cell and developmental biology 8:5955852020CircASH2L Promotes Ovarian Cancer Tumorigenesis, Angiogenesis, and Lymphangiogenesis by Regulating the miR-665/VEGFA Axis as a Competing Endogenous RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinxin Chen et. al.PubMed 33330483
- Pharmaceutics 12:2020Anti-Cancer Activity of AsO and its Efficacy in a Series of Patient-Derived Xenografts for Human Cervical Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph J Noh et. al.PubMed 33086573
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P35968
Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1
Recommended products
Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) is a rabbit polyclonal antibody that is used to detect VEGF Receptor 2 in Western Blot. Suitable for Human samples.
- Over 30 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P35968
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR 2. The final product is generated by affinity chromatography using a VEGFR 2 derived peptide that is phosphorylated at Tyrosines 1054 and 1059.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.
Trusted by the scientific community
Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) (ab5473) was first used in a scientific publication in 2003 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) (ab5473) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
- Biological function summary
VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.
- Pathways
VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.
- Associated diseases and disorders
VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473)
A 150 kDa band corresponding to VEGFR (pYpY1054/1059) was observed across cell lines tested.
Protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard.
Resolved proteins were then transferred onto a nitrocellulose membrane by over night wet transfer.
Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
All lanes: Western blot - Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) at 1/1000 dilution
Lanes 1 - 4: Goat anti-rabbit IgG HRP conjugate at 1/5000 dilution with Skimmed milk
Lane 1: HUVEC (Human umbilical vein endothelial cell line) whole cell extract at 30 µg with Skimmed milk
Lane 2: HUVEC cells treated with 25ng/ml VEGF for 5 minutes at 30 µg with Skimmed milk
Lane 3: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract at 30 µg with Skimmed milk
Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg with Skimmed milk
Predicted band size: 151 kDa
Immunocytochemistry - Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473)
Immunofluorescence analysis of HUVEC cells labelling VEGF Receptor 2 (phospho Y1054 + Y1059) (PAnel a: green) using ab5473 at 2 ug/ml in 0.1% BSA for 3 hours at room temperature, follwed by Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution. Pbel b: Nuclei stained with DAPI (blue), Panel c: F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red), Panel d: Merged images. The images were captured at 60X magnification.
Prior antibody incubation, HUVEC (Human umbilical vein endothelial cell line) cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature, followed by treatment with 100 ng of VEGF for 30 minutes. Assay was done on 90% confluent log phase HUVEC cells.
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