Rabbit Polyclonal VEGF Receptor 2 phospho Y1175 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.
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- Annals of medicine 54:1357-13712022Treatment of malignant pleural effusion in non-small cell lung cancer with VEGF-directed therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhangqiang Xiang,Xiangyu Deng,Wenfeng He,Qian Yang,Laichao Ni,Marzieh Dehghan Shasaltaneh,Mazaher Maghsoudloo,Gang Yang,Jingbo Wu,Saber Imani,Qinglian WenPubMed 35543207
- Frontiers in physiology 12:8077472022Tissue Inhibitor of Metalloproteinase-3 Ameliorates Diabetes-Induced Retinal Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed M Abu El-Asrar,Ajmal Ahmad,Mohd Imtiaz Nawaz,Mohammad Mairaj Siddiquei,Alexandra De Zutter,Lotte Vanbrabant,Priscilla W Gikandi,Ghislain Opdenakker,Sofie StruyfPubMed 35082694
- Cell death discovery 6:1402020FN-EDA mediates angiogenesis of hepatic fibrosis via integrin-VEGFR2 in a CD63 synergetic manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaonan Su et. al.PubMed 33293521
- Stem cell research & therapy 11:5202020Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton's jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Shi et. al.PubMed 33261656
- Experimental and therapeutic medicine 19:1336-13422020Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengtao Wang,Cui Wu,Min Zhang,Aiping Dong,Ruibin Niu,Jie ZhangPubMed 32010307
- EBioMedicine 48:36-482019Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Jiang et. al.PubMed 31631037
- Pathology, research and practice 215:816-8212019Apatinib regulates the cell proliferation and apoptosis of liver cancer by regulation of VEGFR2/STAT3 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Wen et. al.PubMed 30686547
- Scientific reports 8:179242018A peptide mimicking the binding sites of VEGF-A and VEGF-B inhibits VEGFR-1/-2 driven angiogenesis, tumor growth and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam Farzaneh Behelgardi et. al.PubMed 30560942
- Aging 10:3421-34372018miR-150-5p suppresses tumor progression by targeting VEGFA in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxiang Chen,Xueni Xu,Bei Pan,Kaixuan Zeng,Mu Xu,Xiangxiang Liu,Bangshun He,Yuqin Pan,Huiling Sun,Shukui WangPubMed 30476901
- Journal of the American Heart Association 7:e0076012018Disruption of VEGF Mediated Flk-1 Signaling Leads to a Gradual Loss of Vessel Health and Cardiac Function During Myocardial Infarction: Potential Therapy With Pellino-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMahesh Thirunavukkarasu,Vaithinathan Selvaraju,Mandip Joshi,Vladimir Coca-Soliz,Leonidas Tapias,IbnalWalid Saad,Craig Fournier,Aaftab Husain,Jacob Campbell,Siu-Pok Yee,Juan A Sanchez,J Alexander Palesty,David W McFadden,Nilanjana MaulikPubMed 30371196
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P35968
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
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7 products for Alternative Product
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1
Recommended products
Rabbit Polyclonal VEGF Receptor 2 phospho Y1175 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P35968
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
- Biological function summary
VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.
- Pathways
VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.
- Associated diseases and disorders
VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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2 product images
Western blot - Anti-VEGF Receptor 2 (phospho Y1175) antibody (ab194806)
Blocking buffer: 3% BSA
All lanes: Western blot - Anti-VEGF Receptor 2 (phospho Y1175) antibody (ab194806)
Lane 1: HUVEC treated with VEGF cell lysate at 25 µg
Lane 2: Untreated HUVEC cell lysate at 25 µg
SecondaryAll lanes: Goat Anti-rabbit IgG H+L (HRP) at 1/10000 dilution
Predicted band size: 151 kDa
Observed band size: 250 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-VEGF Receptor 2 (phospho Y1175) antibody (ab194806)
VEGF Receptor 2 (phospho Y1175) western blot using anti-VEGF Receptor 2 (phospho Y1175) antibody ab194806. Publication image and figure legend from Chen, X., Xu, X., et al., 2018, Aging (Albany NY), PubMed 30476901.
ab194806 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab194806 please see the product overview.
miR-150-5p inhibited VEGFA/VEGFR2/Akt/mTOR signaling pathway in CRC. Western blot was used to measure the expression of VEGFA, VEGFR2, p-VEGFR2, Akt, p-Akt, mTOR, p-mTOR in transfected HCT116 and HCT8 cells. GAPDH was used as a loading control. Data are shown as the mean±SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001.
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