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Mouse Monoclonal VEGF Receptor 3 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FLT4.

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Images

Flow Cytometry - Anti-VEGF Receptor 3 antibody [9D9] (AB273147), expandable thumbnail
  • Flow Cytometry - Anti-VEGF Receptor 3 antibody [9D9] (AB273147), expandable thumbnail
  • Western blot - Anti-VEGF Receptor 3 antibody [9D9] (AB273147), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Constituents: PBS

Form
Lyophilized
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human FLT4. The exact immunogen used to generate this antibody is proprietary information. Database link P35916

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1-5 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1-5 µg/mL
Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Alternative names

VEGFR3, FLT4, Vascular endothelial growth factor receptor 3, VEGFR-3, Fms-like tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, FLT-4

Recommended products

Mouse Monoclonal VEGF Receptor 3 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FLT4.

Key facts

Isotype
IgG1
Form
Lyophilized
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human FLT4. The exact immunogen used to generate this antibody is proprietary information. Database link P35916
Clone number
9D9
Purification technique
Affinity purification Protein G
Reconstitution
Reconstitute in water
Concentration
Loading...
Purification notes

Purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VEGF Receptor 3 also known as FLT-4 is a protein essential for lymphatic development and is expressed mainly in lymphatic endothelial cells. This receptor has a molecular mass of approximately 195 kDa. VEGF Receptor 3 belongs to the tyrosine kinase family and binds with high affinity to its ligands VEGF-C and VEGF-D. These interactions lead to the activation of intracellular signaling cascades that promote cell proliferation migration and survival.

Biological function summary

The VEGF Receptor 3 acts as an important regulator of lymphangiogenesis which is the growth of new lymphatic vessels from pre-existing ones. It is not typically part of larger protein complexes but it works closely with its ligands to facilitate the processes associated with lymph vessel formation and function. Proper activity of VEGF Receptor 3 is necessary for maintaining normal lymphatic vessel remodeling and function during development and in some adult tissues.

Pathways

The receptor is involved in the VEGF signaling pathway which plays an important role in vascular and lymphatic development. This pathway intersects with others like the PI3K/AKT pathway a critical mediator of cell growth and survival. VEGF Receptor 3 interacts with related receptors such as VEGF Receptor 2 in these pathways to regulate complex and essential physiological processes.

Associated diseases and disorders

VEGF Receptor 3 has been linked to lymphedema which is swelling due to lymphatic system dysfunction and certain cancers where excessive angiogenesis occurs. Mutations or dysregulations in the receptor's signaling can lead to these conditions. In cancer for instance VEGF Receptor 3 along with its associated protein VEGF-C can promote tumor lymphangiogenesis facilitating metastasis. Antagonists like anti-VEGF therapies target these receptors to inhibit tumor progression and manage these disorders.

Product promise

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3 product images

  • Flow Cytometry - Anti-VEGF Receptor 3 antibody [9D9] (ab273147), expandable thumbnail

    Flow Cytometry - Anti-VEGF Receptor 3 antibody [9D9] (ab273147)

    Flow cytometry analysis of VEGF Receptor 3 using in primary human dermal lymphatic endothelial cells using ab273147 at 5 μg/mL.

  • Flow Cytometry - Anti-VEGF Receptor 3 antibody [9D9] (ab273147), expandable thumbnail

    Flow Cytometry - Anti-VEGF Receptor 3 antibody [9D9] (ab273147)

    Flow cytometry analysis of VEGF Receptor 3 in HDMVEC and HUVEC cells using ab273147 at 5 μg/mL.

  • Western blot - Anti-VEGF Receptor 3 antibody [9D9] (ab273147), expandable thumbnail

    Western blot - Anti-VEGF Receptor 3 antibody [9D9] (ab273147)

    All lanes: Western blot - Anti-VEGF Receptor 3 antibody [9D9] (ab273147) at 0.5 µg/mL

    Lane 1: Recombinant human soluble VEGF Receptor 3, 5 ng

    Lane 2: Recombinant human soluble VEGF Receptor 3, 10 ng

    Lane 3: Recombinant human soluble VEGF Receptor 3, 20 ng

    Lane 4: Recombinant human soluble VEGF Receptor 3, 50 ng

    Lane 5: Recombinant human soluble VEGF Receptor 3, 100 ng

    Predicted band size: 146 kDa

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Product protocols

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