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Mouse Monoclonal VEGFA antibody. Suitable for ICC, WB, sELISA and reacts with Human, Transfected cell lysate - Human, Recombinant fragment - Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VEGFA.

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Images

Immunocytochemistry - Anti-VEGFA antibody [16F1] (AB155944), expandable thumbnail
  • Western blot - Anti-VEGFA antibody [16F1] (AB155944), expandable thumbnail
  • Sandwich ELISA - Anti-VEGFA antibody [16F1] (AB155944), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human VEGFA. Database link P15692

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBsELISA
Human
Tested
Expected
Expected
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Transfected cell lysate - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info
1/20.00000 - 1/100.00000
Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info
1 µg/mL
Notes

Typical dilutions for sandwich ELISA include 1μg/ml for coating and 0.125-0.5μg/ml for detection. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132). To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571).

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

N-VEGFParticipates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).VEGFAGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437).Isoform VEGF165BBinds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Alternative names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

Recommended products

Mouse Monoclonal VEGFA antibody. Suitable for ICC, WB, sELISA and reacts with Human, Transfected cell lysate - Human, Recombinant fragment - Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VEGFA.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human VEGFA. Database link P15692
Clone number

16F1

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.

Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

Associated diseases and disorders

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry - Anti-VEGFA antibody [16F1] (ab155944), expandable thumbnail

    Immunocytochemistry - Anti-VEGFA antibody [16F1] (ab155944)

    Immunocytochemistry analysis of HeLa cells labelling VEGFA with ab155944 at 1/40 dilution. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA for 30 minutes at room temperature. No antibody control (left). A Dylight 488 goat anti-mouse IgG secondary antibody was used as a secondary antibody at 1/1000 dilution for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

  • Western blot - Anti-VEGFA antibody [16F1] (ab155944), expandable thumbnail

    Western blot - Anti-VEGFA antibody [16F1] (ab155944)

    4-12% HCl

    All lanes: Western blot - Anti-VEGFA antibody [16F1] (ab155944) at 1 µg/mL

    Lane 1: VEGFA transfected 293T cell culture supernatant at 0.5 µg

    Lane 2: Recombinant VEGFA protein at 0.5 µg

    Predicted band size: 27 kDa

  • Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944), expandable thumbnail

    Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944)

    The plate was coated with 100 μL ab155944 at 1, 3, 4, 5 7 and 9 μg/mL in DPBS for 12 - 18 hours at 4°C. After washing the plate was blocked with 300 μL per well of 4% BSA / 5% sucrose in DPBS for 1 hour at room temperature.

    Recombinant human VEGF protein (50 μL /well) was added at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature.

    After washing the plate was incubated with ab155944 at 0.5 μg /Ml for 1 hour at room temperature.

    Detection was with Streptavidin-HRP / TMB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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