AB155944

Anti-VEGFA antibody [16F1]

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(13 Publications)

Mouse Monoclonal VEGFA antibody. Suitable for ICC, WB, sELISA and reacts with Human, Transfected cell lysate - Human, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VEGFA.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

3 Images

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Immunocytochemistry - Anti-VEGFA antibody [16F1] (AB155944)

Immunocytochemistry analysis of HeLa cells labelling VEGFA with ab155944 at 1/40 dilution. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA for 30 minutes at room temperature. No antibody control (left). A Dylight 488 goat anti-mouse IgG secondary antibody was used as a secondary antibody at 1/1000 dilution for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

Sandwich ELISA - Anti-VEGFA antibody [16F1] (AB155944)

sELISA

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Sandwich ELISA - Anti-VEGFA antibody [16F1] (AB155944)

The plate was coated with 100 μL ab155944 at 1, 3, 4, 5 7 and 9 μg/mL in DPBS for 12 - 18 hours at 4°C. After washing the plate was blocked with 300 μL per well of 4% BSA / 5% sucrose in DPBS for 1 hour at room temperature.

Recombinant human VEGF protein (50 μL /well) was added at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature.

After washing the plate was incubated with ab155944 at 0.5 μg /Ml for 1 hour at room temperature.

Detection was with Streptavidin-HRP / TMB.

Supplier Data

Western blot - Anti-VEGFA antibody [16F1] (AB155944)

4-12% HCl

All lanes:

Western blot - Anti-VEGFA antibody [16F1] (ab155944) at 1 µg/mL

Lane 1:

VEGFA transfected 293T cell culture supernatant at 0.5 µg

Lane 2:

Recombinant VEGFA protein at 0.5 µg

Predicted band size: 27 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

16F1

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, sELISA, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VEGFA.

P15692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment - Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "Typical dilutions for sandwich ELISA include 1μg/ml for coating and 0.125-0.5μg/ml for detection. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (<a href='/en-us/products/primary-antibodies/biotin-vegfa-antibody-ab83132'>ab83132</a>). To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (<a href='/en-us/products/proteins-peptides/recombinant-human-vegf-165a-protein-active-ab9571'>ab9571</a>)." }, "Transfected cell lysate - Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.

Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:923 PubMed40826409

2025

Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodie Liu,Heming Sun,Jing Liang,Huan Yu,Ming Xue,Yuebo Li,Wenhui Li,Ying Xu,Bin Ling,Dingqing Feng

Clinics (Sao Paulo, Brazil) 80:100697 PubMed40466438

2025

CircHIPK3's dual role in promoting angiogenesis and inhibiting apoptosis through FASN mRNA stabilization in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

ZhenWei Li,HaiJiu Wang,Cheng Wang,ZhiXin Wang,Zhi Xie,JinMing Liu

PeerJ 12:e17534 PubMed38948219

2024

β-asarone induces viability and angiogenesis and suppresses apoptosis of human vascular endothelial cells after ischemic stroke by upregulating vascular endothelial growth factor A.

Applications

Unspecified application

Species

Unspecified reactive species

Dazhong Sun,Lulu Wu,Siyuan Lan,Xiangfeng Chi,Zhibing Wu

Nature communications 14:7856 PubMed38030636

2023

A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjian Huang,Rong Yang,Jiao Jiao,Ze Li,Penghui Wang,Ye Liu,Sicheng Li,Canwen Chen,Zongan Li,Guiwen Qu,Kang Chen,Xiuwen Wu,Bo Chi,Jianan Ren

Experimental and therapeutic medicine 24:467 PubMed35747159

2022

PTP1B inhibition ameliorates inflammatory injury and dysfunction in ox-LDL-induced HUVECs by activating the AMPK/SIRT1 signaling pathway via negative regulation of KLF2.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfeng Zhang,Qiang Guan,Zhenfeng Wang

Bioengineered 13:11469-11486 PubMed35535400

2022

Heterogeneous nuclear ribonucleoprotein U-actin complex derived from extracellular vesicles facilitates proliferation and migration of human coronary artery endothelial cells by promoting RNA polymerase II transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wang,Hengdao Liu,Xi Zhao,Xiaowei Chen

Journal of cellular biochemistry : PubMed30485520

2018

MicroRNA-9 inhibits retinal neovascularization in rats with diabetic retinopathy by targeting vascular endothelial growth factor A.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Lan Liu

Oncology letters 16:2207-2214 PubMed30008920

2018

MicroRNA-205 is downregulated in hepatocellular carcinoma and inhibits cell growth and metastasis via directly targeting vascular endothelial growth factor A.

Applications

Unspecified application

Species

Unspecified reactive species

Xuya Zhao,Shi Zhou,Dazhi Wang,Wei He,Junxiang Li,Shuai Zhang

Oncology reports 40:589-598 PubMed29845255

2018

miR‑1 inhibits the progression of colon cancer by regulating the expression of vascular endothelial growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Dehua Zhu,Yefei Sun,Danhua Zhang,Ming Dong,Guiyang Jiang,Xiupeng Zhang,Jianping Zhou

BioMed research international 2018:3145689 PubMed29770329

2018

AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yu,Yan Wang,Huilei Qiu,Hongtao Song,Di Feng,Yang Jiang,Shuzhe Deng,Hongxue Meng,Jingshu Geng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGFA antibody [16F1]

Immunocytochemistry - Anti-VEGFA antibody [16F1] (AB155944)

Sandwich ELISA - Anti-VEGFA antibody [16F1] (AB155944)

Western blot - Anti-VEGFA antibody [16F1] (AB155944)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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