AB39250

Anti-VEGFA antibody

(1 Review)

(33 Publications)

Rabbit Polyclonal VEGFA antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 33 publications.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (AB39250)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (AB39250)

ICC/IF image of ab39250 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39250, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.

Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:6 PubMed39800730

2025

TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Diqing Ouyang,Lin Li,Yunchuan Cao,Yingwen Wang,Nina Gu,Zhaosi Zhang,Zhao Li,Shuang Tang,Hui Tang,Yuan Zhang,Xiaochuan Sun,Jin Yan

Heliyon 10:e37169 PubMed39309801

2024

Asiaticoside inhibits breast cancer progression and tumor angiogenesis via YAP1/VEGFA signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Guo,Yu Ying,Yun Chen,Xian Miao,Zhenghong Yu

Pharmaceutics 15: PubMed38140022

2023

In Vitro Wound Healing Potential of a Fibroin Film Incorporating a Cannabidiol/2-Hydroxypropyl-β-cyclodextrin Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Thamonphat Klinsang,Pensri Charoensit,Preeyawass Phimnuan,Kunlathida Luangpraditkun,Gareth M Ross,Céline Viennet,Sukunya Ross,Jarupa Viyoch

Frontiers in pharmacology 13:1044115 PubMed36467048

2022

Curcumol repressed cell proliferation and angiogenesis SP1/mir-125b-5p/VEGFA axis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Changju Ma,Xiaojuan Tang,Qing Tang,Shiyan Wang,Junhong Zhang,Yue Lu,Jingjing Wu,Ling Han

BMC nephrology 23:313 PubMed36114523

2022

VEGFA promotes the occurrence of PLA2R-associated idiopathic membranous nephropathy by angiogenesis via the PI3K/AKT signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ben Ke,Jinjing Huang,Zhibing Duan,Wen Shen,Yao Wu,Weiping Tu,Xiangdong Fang

Oxidative medicine and cellular longevity 2022:6422202 PubMed36035227

2022

Macrophage Infiltration Reduces Neurodegeneration and Improves Stroke Recovery after Delayed Recanalization in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwei Pang,Nathanael Matei,Jianhua Peng,Wen Zheng,Jing Yu,Xu Luo,Richard Camara,Ligang Chen,Jiping Tang,John H Zhang,Yong Jiang

Neuro-oncology advances 4:vdac123 PubMed36225651

2022

A multi-institutional phase II trial of bevacizumab for recurrent and refractory meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Kumthekar,Sean Aaron Grimm,Roxanne T Aleman,Marc C Chamberlain,David Schiff,Patrick Y Wen,Fabio Massaiti Iwamoto,Demirkan Besim Gursel,David A Reardon,Benjamin Purow,Masha Kocherginski,Irene Helenowski,Jeffrey J Raizer

CNS neuroscience & therapeutics 28:1790-1799 PubMed35855611

2022

Zinc improves neurological recovery by promoting angiogenesis via the astrocyte-mediated HIF-1α/VEGF signaling pathway in experimental stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Tingting Ma,Xiaoyu Zhu,Mingqi Zhang,Liang Zhao,Peng Wang,Jia Liang

Investigative ophthalmology & visual science 63:3 PubMed35816047

2022

Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Hanhan Liu,Katharina Bell,Anja Herrmann,Stefan Arnhold,Karl Mercieca,Fabian Anders,Kerstin Nagel-Wolfrum,Solon Thanos,Verena Prokosch

iScience 25:104651 PubMed35811849

2022

Direct reprogramming of adult adipose-derived regenerative cells toward cardiomyocytes using six transcriptional factors.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Narita,Kazumasa Unno,Katsuhiro Kato,Yusuke Okuno,Yoshitaka Sato,Yusuke Tsumura,Yusuke Fujikawa,Yuuki Shimizu,Ryo Hayashida,Kazuhisa Kondo,Rei Shibata,Toyoaki Murohara

View all publications

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