Rabbit Polyclonal VEGFA antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 26 publications.
Images
Publications
Filter by
- Pharmaceutics 15:2023In Vitro Wound Healing Potential of a Fibroin Film Incorporating a Cannabidiol/2-Hydroxypropyl-β-cyclodextrin Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThamonphat Klinsang,Pensri Charoensit,Preeyawass Phimnuan,Kunlathida Luangpraditkun,Gareth M Ross,Céline Viennet,Sukunya Ross,Jarupa ViyochPubMed 38140022
- Frontiers in pharmacology 13:10441152022Curcumol repressed cell proliferation and angiogenesis SP1/mir-125b-5p/VEGFA axis in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangju Ma,Xiaojuan Tang,Qing Tang,Shiyan Wang,Junhong Zhang,Yue Lu,Jingjing Wu,Ling HanPubMed 36467048
- BMC nephrology 23:3132022VEGFA promotes the occurrence of PLA2R-associated idiopathic membranous nephropathy by angiogenesis via the PI3K/AKT signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBen Ke,Jinjing Huang,Zhibing Duan,Wen Shen,Yao Wu,Weiping Tu,Xiangdong FangPubMed 36114523
- Neuro-oncology advances 4:vdac1232022A multi-institutional phase II trial of bevacizumab for recurrent and refractory meningioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriya Kumthekar,Sean Aaron Grimm,Roxanne T Aleman,Marc C Chamberlain,David Schiff,Patrick Y Wen,Fabio Massaiti Iwamoto,Demirkan Besim Gursel,David A Reardon,Benjamin Purow,Masha Kocherginski,Irene Helenowski,Jeffrey J RaizerPubMed 36225651
- CNS neuroscience & therapeutics 28:1790-17992022Zinc improves neurological recovery by promoting angiogenesis via the astrocyte-mediated HIF-1α/VEGF signaling pathway in experimental stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Li,Tingting Ma,Xiaoyu Zhu,Mingqi Zhang,Liang Zhao,Peng Wang,Jia LiangPubMed 35855611
- Investigative ophthalmology & visual science 63:32022Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanhan Liu,Katharina Bell,Anja Herrmann,Stefan Arnhold,Karl Mercieca,Fabian Anders,Kerstin Nagel-Wolfrum,Solon Thanos,Verena ProkoschPubMed 35816047
- Veterinary pathology 59:940-9492022Effect of porcine reproductive and respiratory syndrome virus 2 on angiogenesis and cell proliferation at the maternal-fetal interface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJavier A Barrera-Zarate,Susan E Detmer,J Alex Pasternak,Glenn Hamonic,Daniel J MacPhee,John C S HardingPubMed 35723036
- Open medicine (Warsaw, Poland) 17:353-3642022miR-29c-5p knockdown reduces inflammation and blood-brain barrier disruption by upregulating LRP6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQijun Dai,Jian Sun,Tianyi Dai,Qin Xu,Yueqin DingPubMed 35799601
- Frontiers in medicine 8:7100792021Reduced Expression of Erythropoietin After Intravitreal Ranibizumab in Proliferative Diabetic Retinopathy Patients-Retrospective Interventional Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,Jing Feng,Yanhong Shi,Fuxiao Luan,Fang Ma,Yingjie Wang,Weiqiang Yang,Yong TaoPubMed 34621759
- Heliyon 7:e065302021Conditioned medium from mesenchymal stem cells improves condylar resorption induced by mandibular distraction osteogenesis in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWataru Katagiri,Satoshi Endo,Ryoko Takeuchi,Daisuke Suda,Naoaki Saito,Tadaharu KobayashiPubMed 33786402
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Expected | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
N-VEGFParticipates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).VEGFAGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437).Isoform VEGF165BBinds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Alternative names
VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF
Recommended products
Rabbit Polyclonal VEGFA antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 26 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
- Biological function summary
VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.
- Pathways
Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.
- Associated diseases and disorders
VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (ab39250)
ICC/IF image of ab39250 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39250, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com