Rabbit Polyclonal VEGFA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant fragment - Mouse, Recombinant fragment - Human, Mouse, Human samples. Cited in 70 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Vegfa.
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Publications
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- Journal of translational medicine 21:8572023Hypoxia-inducible factor 1α modulates interstitial pneumonia-mediated lung cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiyofumi Shimoji,Taku Nakashima,Takeshi Masuda,Masashi Namba,Shinjiro Sakamoto,Kakuhiro Yamaguchi,Yasushi Horimasu,Takahiro Mimae,Shintaro Miyamoto,Hiroshi Iwamoto,Kazunori Fujitaka,Hironobu Hamada,Morihito Okada,Noboru HattoriPubMed 38012636
- Biomedicines 11:2023Neuroprotective Effects of Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Extracellular Vesicles in Ischemic Stroke Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Lu,Xianwei Su,Lihong Wang,Chi-Kwan Leung,Jingye Zhou,Zhiqiang Xiong,Wuming Wang,Hongbin Liu,Wai-Yee ChanPubMed 37760991
- Journal of materials science. Materials in medicine 34:422023Cobalt-containing borate bioactive glass fibers for treatment of diabetic wound.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinhui Zhang,Aihua Yao,Fanrong Ai,Jian Lin,Qingge Fu,Deping WangPubMed 37530851
- Cardiovascular diagnosis and therapy 12:289-3042022TXNIP knockout improves cardiac function after myocardial infarction by promoting angiogenesis and reducing cardiomyocyte apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Wang,Xue-Jiao Wang,Yan Zhang,Wen-Juan Shi,Zhan-Dong Lei,Xiang-Ying JiaoPubMed 35800356
- Annals of translational medicine 10:4462022Maternal obesity inhibits placental angiogenesis by down-regulating the SIRT1/PGC-1α pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuilin Peng,Ming Wei,Lianjing Huang,Yaqin Yan,Qing Li,Sujuan Li,Yichi Wu,Yuan Gao,Ying Xing,Xiaoping LuoPubMed 35571425
- PloS one 17:e02657172022Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnoch Odame Anto,David Antony Coall,Emmanuel Akomanin Asiamah,Osei-Owusu Afriyie,Otchere Addai-Mensah,Yaw Amo Wiafe,Wkba Owiredu,Christian Obirikorang,Max Efui Annani-Akollor,Nicholas Akinwale Titiloye,Eric Adua,Emmanuel Acheampong,Evans Asamoah Adu,Stephen Opoku,Agartha Odame Anto,Augustine Tawiah,Youxin Wang,Wei WangPubMed 35312727
- Cells 10:2021MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Wen Tsai,Kuo-Shyang Jeng,Mu-Kuang He,Yang-Wen Hsieh,Hsin-Hung Lai,Chi-Yu Lai,Chun-Chieh Huang,Chiung-Fang Chang,Chung-Tsui Huang,Guor Mour HerPubMed 34943942
- International journal of molecular sciences 22:2021Thrombin Inhibition Prevents Endothelial Dysfunction and Reverses 20-HETE Overproduction without Affecting Blood Pressure in Angiotensin II-Induced Hypertension in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Kij,Anna Bar,Kamil Przyborowski,Bartosz Proniewski,Lukasz Mateuszuk,Agnieszka Jasztal,Anna Kieronska-Rudek,Brygida Marczyk,Karolina Matyjaszczyk-Gwarda,Anna Tworzydlo,Camilla Enggaard,Pernille B Lærkegaard Hansen,Boye Jensen,Maria Walczak,Stefan ChlopickiPubMed 34445374
- Physiological reports 9:e148632021Intermittent hypoxia changes the interaction of the kinin-VEGF system and impairs myocardial angiogenesis in the hypertrophic heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruna Visniauskas,Juliana C Perry,Guiomar N Gomes,Amanda Nogueira-Pedro,Edgar J Paredes-Gamero,Sergio Tufik,Jair R ChagasPubMed 33991464
- Cancer management and research 13:1477-14892021Long Non-Coding RNA JPX Contributes to Tumorigenesis by Regulating miR-5195-3p/VEGFA in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanglian Li,Xinrui Li,Chao Yuan,Caifeng Zhou,Xinxin Li,Jinfang Li,Bin GuoPubMed 33613033
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Vegfa. Database link Q00731
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
| Recombinant fragment - Human | Tested | Not recommended |
| Recombinant fragment - Mouse | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info 0.1-1 µg/mL | Notes The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Species Recombinant fragment - Human | Dilution info 0.1-1 µg/mL | Notes The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2-10 µg/mL | Notes - |
Species Human | Dilution info 2-10 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity). VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437). Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Alternative names
VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF
Recommended products
Rabbit Polyclonal VEGFA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant fragment - Mouse, Recombinant fragment - Human, Mouse, Human samples. Cited in 70 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Vegfa.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Vegfa. Database link Q00731
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Reconstitution
- The lyophilized IgG is stable at 4°C for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
- Biological function summary
VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.
- Pathways
Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.
- Associated diseases and disorders
VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)
Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.
Top panel: Mouse E17: ventricular zone of the CNS.
Bottom panel: Human glioblastoma: perinecrotic area.
The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany
Western blot - Anti-VEGFA antibody (ab51745)
All lanes: Western blot - Anti-VEGFA antibody (ab51745)
Lane 1: recombinant mouse VEGF164
Lane 2: Recombinant human VEGF165
Lane 3: Recombinant human PlGF-1
Lane 4: Recombinant rat VEGF-C
Predicted band size: 27 kDa
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