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AB214424

Anti-VEGFA antibody [EPR20705]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

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(133 Publications)

Anti-VEGFA antibody [EPR20705] (ab214424) is a rabbit monoclonal antibody detecting VEGFA in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images
Western blot - Anti-VEGFA antibody [EPR20705] (AB214424)
  • WB

Supplier Data

Western blot - Anti-VEGFA antibody [EPR20705] (AB214424)

Blocking/Dilution : 5% NFDM/TBST

Exposure time : Lanes 1-4/7-8 : 3 minutes; Lane 5 : 41 seconds; Lane 6 : 15 seconds.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-VEGFA antibody [EPR20705] (ab214424) at 1/1000 dilution

Lane 1:

Human fetal vessel lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 6:

bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

Lane 7:

C6 (rat glial tumor cell line) whole cell lysate at 20 µg

Lane 8:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

Observed band size: 40 kDa

true

  • Carrier free

    Anti-VEGFA antibody [EPR20705] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20705

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-VEGFA antibody [EPR20705] (ab214424) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of VEGFA?
Anti-VEGFA [EPR20705] (ab214424) specifically detects a band for VEGFA (UniProt: P15692) at a molecular weight of 27kDa.

Trusted by the scientific community
Anti-VEGFA [EPR20705] (ab214424) was first used in a scientific publication in 2017 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20705] also available for your convenience: ab214424, Carrier free - ab229377

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
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Publications (133)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:648 PubMed41074162

2025

Fe-MOF loaded with capmatinib to reverse immunosuppressive microenvironment and inhibit tumor metastasis after radiofrequency ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinchao Zhao,Lei Lei,Cheng Zeng,Xianfei Zhou,Junkang Ding,Wenbin Dai,Angfeng Jiang,Shaomin Zou,Qiao Jin,Zhe Tang

Translational stroke research 16:2211-2231 PubMed40956561

2025

CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Gu,Yong Cai

Frontiers in cardiovascular medicine 12:1633438 PubMed40894482

2025

Transcription factor ETV1 promotes angiogenesis after myocardial infarction via activation of the VEGFA/VEGFR2/eNOS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Wang,Chunxia Li,Feng Li,Sen Fang,Yuan Chen

Pharmaceuticals (Basel, Switzerland) 18: PubMed40872544

2025

Pharmacological Investigation of Tongqiao Jiuxin Oil Against High-Altitude Hypoxia: Integrating Chemical Profiling, Network Pharmacology, and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamei Xie,Yang Yang,Yuhang Du,Xiaohua Su,Yige Zhao,Yongcheng An,Xin Mao,Menglu Wang,Ziyi Shan,Zhiyun Huang,Shuchang Liu,Baosheng Zhao

Nutrients 17: PubMed40805997

2025

Toxigenomic Evaluation of Diallyl Disulfide Effects and Its Association with the Chemotherapeutic Agent 5-Fluorouracil in Colorectal Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Estefani Maria Treviso,Caroline Andolfato Sanchez,Cecília Cristina Souza Rocha,Alexandre Ferro Aissa,Lusânia Maria Greggi Antunes

Reproductive sciences (Thousand Oaks, Calif.) 32:2970-2984 PubMed40789984

2025

Kuntai Capsules Improve Premature Ovarian Failure by Regulating AMPK-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Ye,Miao Chen,Jiajing Zhong,Haofan Chen,Xinmiao Lin

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

Materials today. Bio 33:102016 PubMed40677390

2025

Multifunctional hydrogel promotes rotator cuff healing through anti-inflammation and vascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Bitao Wang,Yiyang Hou,Xi Shang,Yuxuan Zhou,Yubiao Yang,Zhenhan Li,Boyuan Ma,Zhi Zeng,Jinyu Chen,Cheng Tang,Jian Hao,Lianyong Wang,Xianhu Zhou

Journal of cell communication and signaling 19:e70018 PubMed40584821

2025

Hypoxic adipose-derived stem cell exosomes as carriers of miR-100-5p to enhance angiogenesis and suppress inflammation in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Liu,Fei Hao,Bangtao Chen

Endokrynologia Polska 76:290-300 PubMed40586405

2025

BFGF downregulates endoplasmic reticulum stress to alleviate HUVEC cell damage under high-glucose and inflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Hu,Wei Shen,Longyang Xu,Hongming Xu
View all publications
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Product promise

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