AB312862

Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal VEGFA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

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Sandwich ELISA - Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector) (AB312862)

sELISA

Supplier Data

Sandwich ELISA - Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector) (AB312862)

Sandwich ELISA of ab312860 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24630-90

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF or Vascular Endothelial Growth Factor is a signaling protein that plays an essential role in blood vessel formation. Known also as VEGFA its molecular mass is approximately 45 kDa. VEGF is expressed in a variety of tissues especially those with high angiogenic activity such as endothelial cells macrophages and platelets. This protein activates receptors on the surface of endothelial cells triggering a cascade of intracellular events that lead to cellular proliferation migration and increased vascular permeability.

Biological function summary

This protein regulates angiogenesis the process through which new blood vessels form from pre-existing vasculature. It interacts with specific tyrosine kinase receptors such as VEGFR-1 and VEGFR-2. VEGF is not part of a multi-protein complex but it can form homodimers which significantly enhances its activity. It ensures proper vascularization during development and wound healing by directing the growth and branching of vessels. This makes it an important factor in supplying oxygen and nutrients to tissues.

Pathways

VEGF is primarily involved in the VEGF signaling and PI3K/AKT pathways. These pathways allow it to mediate endothelial cell function and survival. In the VEGF signaling pathway it associates closely with members like VEGFR-2 and PLCγ1 which activate further signal transduction needed for angiogenesis. The PI3K/AKT pathway linked with cell proliferation and survival also interacts with VEGF connecting it to other important proteins like AKT and mTOR highlighting its significant role in cellular responses to environmental stimuli.

VEGF has a strong association with cancer and age-related macular degeneration. In cancer overexpression of VEGF leads to pathological angiogenesis providing tumors with the blood supply needed for growth and metastasis. Tumor cells through hypoxia-inducible factors (HIF) upregulate VEGF expression correlating with cancer progression. In age-related macular degeneration abnormal blood vessel growth influenced by VEGF contributes to visual impairment and blindness further implicating the protein in ocular diseases. Targeted therapies often aim at inhibiting VEGF activity to control the disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector) (AB312862)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise