AB110649

Anti-VEGFB antibody [EPR4555]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal VEGFB antibody. Suitable for WB and reacts with Recombinant fragment, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

VRF, VEGFB, Vascular endothelial growth factor B, VEGF-B, VEGF-related factor

1 Images

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Western blot - Anti-VEGFB antibody [EPR4555] (AB110649)

All lanes:

Western blot - Anti-VEGFB antibody [EPR4555] (ab110649) at 1/1000 dilution

All lanes:

VEGFB recombinant protein at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 19 kDa

false

Carrier free

Anti-VEGFB antibody [EPR4555] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4555

Isotype

IgG

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGFB also known as vascular endothelial growth factor B is a member of the VEGF family. It has a molecular mass of approximately 34-35 kDa. VEGFB interacts with its receptors to promote angiogenesis increasing blood supply to tissues. Expression of VEGFB occurs in the heart skeletal muscle and various endothelial cells. It also shows expression in tissues undergoing high metabolic activities. Besides its angiogenic role VEGFB exhibits participation in metabolic regulation.

Biological function summary

VEGFB supports angiogenesis by promoting endothelial cell growth and migration. It acts as a signal protein not forming part of a larger complex. VEGFB binds to VEGFR-1 (Flt-1) receptor which is involved in transmitting the growth-promoting signals. Its function extends to the regulation of fatty acid uptake into tissues highlighting its role in lipid metabolism and energy utilization.

Pathways

The signalling activities of VEGFB are significant in angiogenesis and metabolic pathways. VEGFB interacts closely with the VEGF-A pathway which is vital for forming new blood vessels. It also shares interactive roles with similar proteins such as VEGF-C and VEGF-D contributing to various angiogenic processes and cellular growth signalling systems.

Understanding VEGFB's role is important in conditions like cardiovascular disease and metabolic syndrome. Altered VEGFB signalling may link to heart diseases due to its regulatory function in maintaining heart vasculature. VEGFB has connections with VEGFR-1 and VEGF-A indicating its potential involvement in endothelial dysfunction and highlighting its significance in therapeutic strategies targeting such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

See full target information VEGFB

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of hepatocellular carcinoma 10:1639-1657 PubMed37791068

2023

A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangjin Huang,Jun Xu,Chunming Xie,Yuejuan Liao,Rong Lin,Yuan Zeng,Fujun Yu

Cell cycle (Georgetown, Tex.) 22:331-346 PubMed36200131

2022

Upregulated miR-194-5p suppresses retinal microvascular endothelial cell dysfunction and mitigates the symptoms of hypertensive retinopathy in mice by targeting SOX17 and VEGF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Wan,Heting Liu,Yuxin Xu,Qing Zhang,Liming Tao

Cancer cell international 22:221 PubMed35773697

2022

VEGF-B targeting by aryl hydrocarbon receptor mediates the migration and invasion of choriocarcinoma stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qianxia Tan,Jingting Cai,Jingping Peng,Cui Hu,ChenChun Wu,Huining Liu

Cancers 14: PubMed35267513

2022

Impact of Exogenous Treatment with Histidine on Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yusun Park,Yeonju Han,Dongwoo Kim,Sua Cho,WonJin Kim,Hyemin Hwang,Hye Won Lee,Dai Hoon Han,Kyung Sik Kim,Mijin Yun,Misu Lee

PeerJ 9:e11009 PubMed33763303

2021

Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Wang,Ying Feng,Wen-Jing Zhou,Zhao-Jun Cheng,Mei-Yan Jiang,Yan Zhou,Xiao-Yang Fei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGFB antibody [EPR4555]

Western blot - Anti-VEGFB antibody [EPR4555] (AB110649)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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