AB9546

Anti-VEGFC antibody

Name: Anti-VEGFC antibody. Rabbit polyclonal (ab9546) | Abcam
Brand: Abcam Limited
SKU: AB9546
Price: 505 USD
Availability: InStock

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(43 Publications)

Rabbit Polyclonal VEGFC antibody. Carrier free. Suitable for ELISA, WB, IHC-FoFr, IHC-P and reacts with Recombinant full length protein - Rat, Recombinant full length protein - Human, Sheep, Mouse, Rat, Human samples. Cited in 43 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Vegfc aa 100-250.

View Alternative Names

Vascular endothelial growth factor C, VEGF-C, Flt4 ligand, Vascular endothelial growth factor-related protein, Flt4-L, VRP, VEGFC

5 Images

ELISA

Supplier Data

ELISA - Anti-VEGFC antibody (AB9546)

VEGF-C sandwich-ELISA using ab9546 as capture antibody and recombinant rat VEGF-C as standard. Biotinylated rabbit anti-rat VEGF-C was used for detection.

Unknown

Western blot - Anti-VEGFC antibody (AB9546)

Western blot with human and rat VEGF C and VEGF D using the polyclonal anti VEGF C antibody ab9546. Proteins were separated by 15% SDS-PAGE and blotted on to PVDF membranes as described. After transfer, the membrane was incubated for 1 h with ab9546 at 1 μg/ml in TBS containing 20% non-fat milk followed by incubation with a goat-anti-rabbit alkaline phosphatase-conjugated secondary antibody. Lane 1/250ng rat VEGF C; lane 2/250 ng human VEGF C; lane 3/250 ng rat VEGF D; lane4/250 ng human VEGF-D.

All lanes:

Western blot - Anti-VEGFC antibody (ab9546)

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-VEGFC antibody (AB9546)

Western analysis of recombinant human and rat VEGF-C using a polyclonal rabbit anti-rat VEGF-C antibody.

All lanes:

Western blot - Anti-VEGFC antibody (ab9546)

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-VEGFC antibody (AB9546)

Western blot with recombinant rat VEGF C fused to His-tag using the polyclonal anti VEGF C antibody ab9546. Bands are between 15kDa and 20kDa represents glycosylation forms.

All lanes:

Western blot - Anti-VEGFC antibody (ab9546)

Predicted band size: 46 kDa

false

Unknown

Western blot - Anti-VEGFC antibody (AB9546)

Western Blot was run on a 15% SDS-PAGE gel. Bands between 15kDa and 20kDa represent the different glycoslation forms.

All lanes:

Western blot - Anti-VEGFC antibody (ab9546)

All lanes:

Rat Recombinant VEGF-C insect cells

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse, Sheep

Applications

ELISA, WB, IHC-P, IHC-FoFr

applications

Immunogen

Recombinant Fragment Protein within Rat Vegfc aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O35757

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2-5 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-5 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1-5 µg/mL", "ELISA-species-notes": "Allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-5 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGFC also known as Vascular Endothelial Growth Factor C is a member of the VEGF family involved in angiogenesis and lymphangiogenesis. It has a molecular mass of approximately 35 kDa. VEGFC is expressed in various tissues with high levels in the heart lung and muscle. It acts primarily through its interaction with the VEGFR-3 receptor stimulating the formation of blood and lymphatic vessels. This protein plays an important role in the development and maintenance of the circulatory and lymphatic systems.

Biological function summary

VEGFC contributes to the regulation of vascular and lymphatic endothelial cell functions. It is not known to be part of a larger protein complex but exerts its functions through interactions with receptors such as VEGFR-3. By promoting cell proliferation and migration VEGFC assists in the repair and regeneration of tissues. Its role in fluid balance is essential as it helps maintain tissue equilibrium and immune function.

Pathways

VEGFC is integral to the VEGF signaling and lymphatic vessel development pathways. Within these processes VEGFC works alongside other proteins such as VEGFA and VEGFB to modulate vascular permeability and vessel formation. The interaction between VEGFC and the VEGFR-3 receptor specifically mediates lymphatic endothelial cell activation and function linking it to vital physiological processes like inflammation and tissue repair.

VEGFC is associated with cancer and lymphedema. Its role in promoting angiogenesis is critical in tumor growth and metastasis with its overexpression often linked to cancer progression. Furthermore insufficient VEGFC activity contributes to the development of lymphedema due to inadequate lymphatic drainage. In cancer pathology VEGFC works together with VEGFR-3 contributing to the abnormal growth of lymphatic vessels which in turn facilitates cancer cell dissemination.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.

See full target information VEGFC

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:320 PubMed40651955

2025

Inhibition of VEGFR-3 by SAR131675 decreases renal inflammation and lymphangiogenesis in the murine lupus nephritis model.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Wang,Wenjia Li,Ji-Hyun Yeom,Zhiheng Liu,Kyoung Min Kim,Kyung Pyo Kang

The clinical respiratory journal 18:e13805 PubMed39003635

2024

The Novel Fusion Protein Melittin-MIL-2 Exhibits Strong Antitumor Immune Effect in Lung Adenocarcinoma Cell A549.

Applications

Unspecified application

Species

Unspecified reactive species

Weize Gao,Wenshuai Li,Zhan Wang,Yongxin Li,Mingjun Liu

Anais brasileiros de dermatologia 99:362-369 PubMed38350760

2024

Clinicopathological and immunohistochemical characteristics of bullous pilomatricoma: a retrospective, single-center study, and comparison with ordinary pilomatricoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung-Hwa Nam,Sang-Kyung Lee,Il-Jae Lee,Jin Park,Seok-Kweon Yun

Cell death & disease 14:708 PubMed37903800

2023

The microprotein encoded by exosomal lncAKR1C2 promotes gastric cancer lymph node metastasis by regulating fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Gan Zhu,Jiayu Yang,Yuehong Zhu,Qihang Zhu,Wen Pan,Siyu Deng,Yi He,Duo Zuo,Peiyun Wang,Yueting Han,Hai-Yang Zhang

Molecular medicine reports 28: PubMed37654213

2023

Inhibition of TP signaling promotes endometriosis growth and neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Akiko Furue,Kyoko Hattori,Kanako Hosono,Mina Tanabe,Erina Sato,Masako Honda,Kazuki Sekiguchi,Yoshiya Ito,Masataka Majima,Shuh Narumiya,Kazuyoshi Kato,Hideki Amano

Science translational medicine 15:eadf1128 PubMed37134152

2023

Inhibition of VEGF binding to neuropilin-2 enhances chemosensitivity and inhibits metastasis in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Xu,Hira Lal Goel,Christoph Burkart,Luke Burman,Yeeting E Chong,Alison G Barber,Yanyan Geng,Liting Zhai,Mengdie Wang,Ayush Kumar,Ann Menefee,Clara Polizzi,Lisa Eide,Kaitlyn Rauch,Justin Rahman,Kristina Hamel,Zachary Fogassy,Sofia Klopp-Savino,Suzanne Paz,Mingjie Zhang,Andrea Cubitt,Leslie A Nangle,Arthur M Mercurio

Journal of periodontal research 58:596-606 PubMed36843064

2023

Human gingival epithelial cells stimulate proliferation, migration, and tube formation of lymphatic endothelial cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Stine Hufthammer Indrelid,Harsh Nitin Dongre,Ivana Pereira Nunes,Anca Virtej,Athanasia Bletsa,Ellen Berggreen

BMC urology 22:193 PubMed36434587

2022

NEAT1 promotes the malignant development of bladder cancer by regulating the miR-101/VEGF-C pathway in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Zhang,Shuang Yu,Kuilin Fei,Zhongxin Huang,Shidong Deng,Hanfeng Xu

Experimental and therapeutic medicine 24:623 PubMed36160894

2022

Eplerenone ameliorates lung fibrosis in unilateral ureteral obstruction rats by inhibiting lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqian Liu,Cuijuan Zhang,Juan Hao,Gege Chen,Lingjin Liu,Yunzhao Xiong,Yi Chang,Hui Li,Tatsuo Shimosawa,Fan Yang,Qingyou Xu

Cell death & disease 13:272 PubMed35347106

2022

Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Dong Wang,Xiao-Jun Yu,Ji-Chun Hou,Bo Fu,Hao Zheng,Qi-Kun Liu,Shan-Xi Wang,Zheng-Gang Bi,Yang Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGFC antibody

ELISA - Anti-VEGFC antibody (AB9546)

Western blot - Anti-VEGFC antibody (AB9546)

Western blot - Anti-VEGFC antibody (AB9546)

Western blot - Anti-VEGFC antibody (AB9546)

Western blot - Anti-VEGFC antibody (AB9546)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (43)

Product promise