Anti-VEGFD antibody [EPR8457] (ab155288)

Rabbit Recombinant Monoclonal VEGFD antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 15 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (AB155288), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Expected	Tested
Mouse	Expected	Expected	Tested	Expected
Rat	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000 - 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100 - 1/500	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/700	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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2 products for Alternative Product

Target data

See full target information VEGFD

Function

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

Alternative names

FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor

Recommended products

Rabbit Recombinant Monoclonal VEGFD antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 15 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR8457
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.

Biological function summary

Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.

Pathways

The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.

Associated diseases and disorders

Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)
ab155288 staining VEGFD in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - Formalin/paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control: PBS in place of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-VEGFD antibody [EPR8457] (ab155288)
ab155288 staining VEGFD in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody ab155288 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)
Diluting and blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution
Lane 1: Raji (human Burkitt's lymphoma) whole cell lysate at 20 µg
Lane 2: HT1080 (human fibrosarcoma) whole cell lysate at 20 µg
Lane 3: Caco-2 (human colorectal adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)
Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution
All lanes: GST-Tagged human VEGFD (aa 1 to 354) recombinant protein
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 65 kDa
Flow Cytometry (Intracellular) - Anti-VEGFD antibody [EPR8457] (ab155288)
ab155288 staining VEGFD in the mouse cell line NIH/3T3 (mouse embryo) by intracellular flow cytometry.Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)
Diluting and blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-VEGFD antibody [EPR8457] (ab155288) at 1/2000 dilution
Lane 1: Rat lung tissue lysate at 15 µg
Lane 2: NIH/3T3 (mouse embryo) whole cell lysate at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)
All lanes: Western blot - Anti-VEGFD antibody [EPR8457] (ab155288) at 1/1000 dilution
Lane 1: Raji lysate at 10 µg
Lane 2: HT-1080 lysate at 10 µg
Lane 3: MCF-7 lysate at 10 µg
Lane 4: Caco-2 lysate at 10 µg
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)
Immunohistochemical analysis of paraffin embedded Human heart tissue labeling VEGFD with ab155288 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)
Immunohistochemical analysis of paraffin embedded Human lung tissue labeling VEGFD with ab155288 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)
Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155288 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFD antibody [EPR8457] (ab155288)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab155288showing +ve staining.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-VEGFD antibody [EPR8457] (ab155288)
VEGFD western blot using anti-VEGFD antibody [EPR8457] ab155288. Publication image and figure legend from Hong, H., Jiang, L., et al., 2016, BMC Cancer, PubMed 26992854.

ab155288 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155288 please see the product overview.
TNF-α upregulated VEGF-D expression and VEGF-D promoter activity downstream of the ERK1/2/AP-1 pathway. a, c The effect of the TNF-α⁄AP-1 signaling pathway on the promoter activity and protein expression of the VEGF-D gene. Transfection with AP-1 siRNA effectively knocked down the expression of AP-1 and p-AP-1 in both NOZ and GBC-SD cells. The protein level and promoter activity of VEGF-D were accordingly reduced irrespective of treatment with TNF-α. b, d The effect of inhibition of MAPK pathway members on the protein expression and promoter activity of VEGF-D. When treated with SP600125 (10 μM), SB203580 (20 μM) or PD98059 (50 μM), the expression of AP-1 and p-AP-1 in both NOZ and GBC-SD cells were reduced. However, the protein expression and promoter activity of VEGF-D were significantly reduced only in the PD98059-treated group. *P < 0.05