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AB313855

Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free

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Rabbit Recombinant Monoclonal Venezuelan equine encephalitis virus NSP1 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Transfected cell line - Venezuelan equine encephalitis virus, Venezuelan equine encephalitis virus, Transfected cell lysate - Venezuelan equine encephalitis virus samples.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)

Immunohistochemical analysis of paraffin-embedded mock and Venezuelan equine encephalitis virus nsp1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell pellets labeling Venezuelan equine encephalitis virus nsp1 with ab313855 at 1/1000 dilution (green). Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)

Immunofluorescent analysis of mock and Venezuelan equine encephalitis virus nsp1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Venezuelan equine encephalitis virus nsp1 with ab313855 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)

Immunofluorescent analysis of Multiple virus infected cells labelling Venezuelan Equine Encephalitis Virus nsp1 with ab313855 at 1/100 dilution.

Blue : Fluoroshield with DAPI.

Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)
  • WB

Supplier Data

Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)

All lanes:

Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855) at 1/10000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

DDDDK-tag tagged Venezuelan equine ecenphalitis virus nsp1 transfected HEK293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1472

Isotype

IgG

Carrier free

Yes

Reacts with

Venezuelan equine encephalitis virus

Applications

ICC/IF, WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Venezuelan equine encephalitis virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Venezuelan equine encephalitis virus": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Venezuelan equine encephalitis virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Venezuelan equine encephalitis virus NSP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com