Rabbit Recombinant Monoclonal Venezuelan equine encephalitis virus NSP1 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Transfected cell line - Venezuelan equine encephalitis virus, Venezuelan equine encephalitis virus, Transfected cell lysate - Venezuelan equine encephalitis virus samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--immunocytochemistry-immunofluorescence-img429435.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418913.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)")
![Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--immunocytochemistry-immunofluorescence-img418897.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)")
![Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--western-blot-img418880.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (AB313855)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC/IF | IHC-P | WB | |
|---|---|---|---|
| Transfected cell line - Venezuelan equine encephalitis virus | Tested | Tested | Not recommended |
| Transfected cell lysate - Venezuelan equine encephalitis virus | Not recommended | Not recommended | Tested |
| Venezuelan equine encephalitis virus | Tested | Expected | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Venezuelan equine encephalitis virus | Dilution info 1/500 | Notes - |
Species Venezuelan equine encephalitis virus | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Venezuelan equine encephalitis virus | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Venezuelan equine encephalitis virus | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Venezuelan equine encephalitis virus | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Venezuelan equine encephalitis virus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Venezuelan equine encephalitis virus | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Venezuelan equine encephalitis virus | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Venezuelan equine encephalitis virus | Dilution info - | Notes - |
Rabbit Recombinant Monoclonal Venezuelan equine encephalitis virus NSP1 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Transfected cell line - Venezuelan equine encephalitis virus, Venezuelan equine encephalitis virus, Transfected cell lysate - Venezuelan equine encephalitis virus samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1472
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--immunocytochemistry-immunofluorescence-img429435.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)")
Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)
Immunofluorescent analysis of Multiple virus infected cells labelling Venezuelan Equine Encephalitis Virus nsp1 with ab313855 at 1/100 dilution.
Blue: Fluoroshield with DAPI.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418913.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)
Immunohistochemical analysis of paraffin-embedded mock and Venezuelan equine encephalitis virus nsp1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell pellets labeling Venezuelan equine encephalitis virus nsp1 with ab313855 at 1/1000 dilution (green). Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.
![Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--immunocytochemistry-immunofluorescence-img418897.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)")
Immunocytochemistry/ Immunofluorescence - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)
Immunofluorescent analysis of mock and Venezuelan equine encephalitis virus nsp1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Venezuelan equine encephalitis virus nsp1 with ab313855 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.
![Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855), expandable thumbnail](https://content.abcam.com/products/images/venezuelan-equine-encephalitis-virus-nsp1-antibody-hl1472-bsa-and-azide-free-ab313855--western-blot-img418880.jpg "Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)")
Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855)
All lanes: Western blot - Anti-Venezuelan Equine Encephalitis Virus nsp1 antibody [HL1472] - BSA and Azide free (ab313855) at 1/10000 dilution
Lane 1: Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: DDDDK-tag tagged Venezuelan equine ecenphalitis virus nsp1 transfected HEK293T whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Downloads
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- Visit the general protocols
- Visit troubleshooting
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