Mouse Monoclonal STXA antibody. Suitable for WB and reacts with Escherichia coli, Chemical samples.
Images
![Western blot - Anti-Verotoxin I / SLT 1a antibody [VERO-01] (AB243100), expandable thumbnail](https://content.abcam.com/products/images/verotoxin-i-slt-1a-antibody-vero-01-ab243100--western-blot-img101567.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Verotoxin I / SLT 1a antibody [VERO-01] (AB243100)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 6.5 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | |
|---|---|
| Chemical | Tested |
| Escherichia coli | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info 1/500 - 1/1000 | Notes - |
Species Chemical | Dilution info 1/500 - 1/1000 | Notes - |
Target data
Function
The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.
Alternative names
sltA, stxA, Shiga-like toxin 1 subunit A, SLT-1 A subunit, SLT-1a, SLT-Ia, Verocytotoxin 1 subunit A, Verotoxin 1 subunit A, rRNA N-glycosidase 1
Recommended products
Mouse Monoclonal STXA antibody. Suitable for WB and reacts with Escherichia coli, Chemical samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 6.5 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- VERO-01
- Purification technique
- Affinity purification Protein A
- Specificity
Reacts with subunit A of Verotoxin 1, Verotoxin 2 and Stx
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Verotoxin I / SLT 1a antibody [VERO-01] (ab243100), expandable thumbnail](https://content.abcam.com/products/images/verotoxin-i-slt-1a-antibody-vero-01-ab243100--western-blot-img101567.jpg "Western blot - Anti-Verotoxin I / SLT 1a antibody [VERO-01] (ab243100)")
Western blot - Anti-Verotoxin I / SLT 1a antibody [VERO-01] (ab243100)
Verotoxin I / SLT 1a Western blot staining using mouse Anti-Verotoxin I / SLT 1a antibody
All lanes: Western blot - Anti-Verotoxin I / SLT 1a antibody [VERO-01] (ab243100) at 1/500 dilution
Lane 1: Culture medium of E. coli O157:H7
Lane 2: Purified Verotoxin 1 / SLT 1a
Lane 3: Purified Verotoxin 2
Lane 4: Culture medium from Verotoxin non-producing E. coli
Predicted band size: 35 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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