Anti-Versican antibody [EPR12277] - BSA and Azide free

• WB

Unknown

Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)

Blocking and diluting buffer : 5% NFDM/TBST

This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID : 27683612, 24401530, 29222454, 19662655)

This data was developed using ab177480, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Versican antibody [EPR12277] (<a href='/en-us/products/primary-antibodies/versican-antibody-epr12277-ab177480'>ab177480</a>) at 0.1 µg/mL

Lane 1:

Human hippocampus lysate at 20 µg

Lane 2:

Mouse hippocampus lysates at 20 µg

Lane 3:

Mouse heart lysates at 20 µg

Lane 4:

Rat hippocampus lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 373 kDa

false

• WB

Supplier Data

Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177480).

All lanes:

Western blot - Anti-Versican antibody [EPR12277] (<a href='/en-us/products/primary-antibodies/versican-antibody-epr12277-ab177480'>ab177480</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Predicted band size: 373 kDa

false