Rabbit Recombinant Monoclonal Versican antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200), expandable thumbnail](https://content.abcam.com/products/images/versican-antibody-epr12277-bsa-and-azide-free-ab240200--western-blot-img33093.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)")
![Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200), expandable thumbnail](https://content.abcam.com/products/images/versican-antibody-epr12277-bsa-and-azide-free-ab240200--western-blot-img60778.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Alternative names
CSPG2, VCAN, Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, Chondroitin sulfate proteoglycan 2, GHAP
Recommended products
Rabbit Recombinant Monoclonal Versican antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12277
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240200 is the carrier-free version of Anti-Versican antibody [EPR12277] ab177480.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200), expandable thumbnail](https://content.abcam.com/products/images/versican-antibody-epr12277-bsa-and-azide-free-ab240200--western-blot-img33093.jpg "Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200)")
Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200)
Blocking and diluting buffer: 5% NFDM/TBST
This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655)
This data was developed using Anti-Versican antibody [EPR12277] ab177480, the same antibody clone in a different buffer formulation.All lanes: Western blot - Anti-Versican antibody [EPR12277] (Anti-Versican antibody [EPR12277] ab177480) at 0.1 µg/mL
Lane 1: Human hippocampus lysate at 20 µg
Lane 2: Mouse hippocampus lysates at 20 µg
Lane 3: Mouse heart lysates at 20 µg
Lane 4: Rat hippocampus lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 373 kDa
![Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200), expandable thumbnail](https://content.abcam.com/products/images/versican-antibody-epr12277-bsa-and-azide-free-ab240200--western-blot-img60778.jpg "Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200)")
Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Versican antibody [EPR12277] ab177480).
All lanes: Western blot - Anti-Versican antibody [EPR12277] (Anti-Versican antibody [EPR12277] ab177480) at 1/1000 dilution
Lane 1: Fetal brain lysate at 10 µg
Lane 2: Fetal heart lysate at 10 µg
Predicted band size: 373 kDa
Downloads
Product protocols
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