Anti-Versican antibody [EPR23135-58]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Versican with ab269270 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in tunica propria of human seminiferous tubules (PMID : 8601689). The section was incubated with ab269270 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Versican with ab269270 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebral cortex (PMID : 18936977). The section was incubated with ab269270 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labelling Versican with ab269270 at 1/500 dilution (1.176 μg/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at  dilution. Positive staining on stromal cells of human clear cell carcinoma. The section was incubated with ab269270 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at  dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Versican with ab269270 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in stromal cells of mouse stomach. The section was incubated with ab269270 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Versican with ab269270 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebral cortex. The section was incubated with ab269270 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-Versican antibody [EPR23135-58] (AB269270)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular wight around 370kDa was described : (doi : 10.4172/2329-6917.1000113)

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Versican antibody [EPR23135-58] (ab269270) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 373 kDa

Observed band size: 370 kDa

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