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AB270444

Anti-Versican antibody [EPR23374-21]

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(2 Publications)

Rabbit Recombinant Monoclonal Versican antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

CSPG2, VCAN, Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, Chondroitin sulfate proteoglycan 2, GHAP

1 Images
Western blot - Anti-Versican antibody [EPR23374-21] (AB270444)
  • WB

Lab

Western blot - Anti-Versican antibody [EPR23374-21] (AB270444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : MDA-MB-231(PMID : 29222454); MeWo(PMID : 12972299).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Versican antibody [EPR23374-21] (ab270444) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

MeWo (human malignant melanoma fibroblast) whole cell lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 373 kDa

Observed band size: 370 kDa

false

  • Carrier free

    Anti-Versican antibody [EPR23374-21] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23374-21

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

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Western blot - Anti-Versican antibody [EPR12277] (AB177480)

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Complementary Products

Assay Kits

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Human Versican ELISA Kit

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
See full target information VCAN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:934-948 PubMed40128621

2025

The landscape of N-methyladenosine in localized primary prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Helen Zhu,Rupert Hugh-White,Julie Livingstone,Stefan Eng,Nicole Zeltser,Yujuan Wang,Kinga Pajdzik,Sujun Chen,Kathleen E Houlahan,Wenqin Luo,Shun Liu,Xi Xu,Minzhi Sheng,Wang Yuan Guo,Jaron Arbet,Yuxi Song,Miranda Wang,Yong Zeng,Shiyan Wang,Guanghui Zhu,Tingxiao Gao,Wei Chen,Xinpei Ci,Wenjie Xu,Kexin Xu,Michele Orain,Valerie Picard,Helene Hovington,Alain Bergeron,Louis Lacombe,Bernard Têtu,Yves Fradet,Mathieu Lupien,Gong-Hong Wei,Marianne Koritzinsky,Robert G Bristow,Neil E Fleshner,Xue Wu,Yang Shao,Chuan He,Alejandro Berlin,Theodorus van der Kwast,Hon Leong,Paul C Boutros,Housheng Hansen He

Molecular human reproduction 29: PubMed37774003

2023

Upregulated TIMP1 facilitates and coordinates myometrial contraction by decreasing collagens and cell adhesive capacity during human labor.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Bao,Xiaodi Wang,Lina Chen,Bolun Wen,Qiu Gao,Xiuyu Pan,Yunshan Chen,Kaiyuan Ji,Huishu Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com