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Rabbit Recombinant Monoclonal Versican antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Versican antibody [EPR23374-21] - BSA and Azide free (AB272223), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IFIHC-Fr
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

Alternative names

CSPG2, VCAN, Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, Chondroitin sulfate proteoglycan 2, GHAP

Recommended products

Rabbit Recombinant Monoclonal Versican antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR23374-21
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab272223 is the carrier-free version of Anti-Versican antibody [EPR23374-21] ab270444.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223), expandable thumbnail

    Western blot - Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MDA-MB-231(PMID:29222454); MeWo(PMID:12972299).

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containg PBS, glycerol, BSA and sodium azide (Anti-Versican antibody [EPR23374-21] ab270444).

    All lanes: Western blot - Anti-Versican antibody [EPR23374-21] (Anti-Versican antibody [EPR23374-21] ab270444) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Human brain tissue lysate at 20 µg

    Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: MeWo (human malignant melanoma fibroblast) whole cell lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 373 kDa

    Observed band size: 370 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com