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AB325671

Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric)

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Chicken Monoclonal VGluT1 antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Bnpi, Vglut1, Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (AB325671)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (AB325671)

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Vesicular glutamate transporter 1. The sections were stained with ab325671 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the axonal synapses in the granular layer of the cerebellum. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (AB325671)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (AB325671)

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Vesicular glutamate transporter 1. The sections were stained with ab325671 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the axonal synapses in the granular layer of the cerebellum. Magnification 100X.

Western blot - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (AB325671)
  • WB

Supplier Data

Western blot - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (AB325671)

Western blotting of rat brain membrane fraction with ab325671 antibody at various concentrations and detected with anti-chicken-HRP. The antibody recognizes endogenous Vesicular glutamate transporter 1 in the rat brain membrane fraction at 52 kDa.

Lane 1:

Western blot - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (ab325671) at 10 µg/mL

Lane 2:

Western blot - Anti-Vesicular glutamate transporter 1 antibody [N28/9] - Chicken IgY (Chimeric) (ab325671) at 20 µg/mL

All lanes:

rat brain membrane fraction

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 52 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

N28/9

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Does not cross-react with VGlut2 or VGlut3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10-20 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N28/9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N28/9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 10938000, PubMed : 11001057, PubMed : 11698619, PubMed : 18080752, PubMed : 19169251, PubMed : 25433636, PubMed : 27133463, PubMed : 29642010, PubMed : 33440152, PubMed : 8202535). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed : 10938000, PubMed : 11001057, PubMed : 11698619, PubMed : 18080752, PubMed : 29642010). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed : 29642010). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (PubMed : 10938000, PubMed : 19169251, PubMed : 25433636, PubMed : 27133463, PubMed : 29642010). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (PubMed : 25433636). The vesicular K(+)/H(+) antiport activity is electroneutral (PubMed : 25433636). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed : 29642010, PubMed : 33440152, PubMed : 8202535). The symporter activity is driven by an inside negative membrane potential and is electrogenic (PubMed : 29642010). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity).
See full target information Slc17a7
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Product promise

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