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Rabbit Recombinant Monoclonal NALCN antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (AB323186), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (AB323186), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (AB323186), expandable thumbnail
  • Western blot - Anti-VGCNL1 antibody [EPR29666-91] (AB323186), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (AB323186), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Voltage-gated ion channel responsible for the resting Na(+) permeability that controls neuronal excitability (PubMed:17448995, PubMed:31409833). NALCN channel functions as a multi-protein complex, which consists at least of NALCN, NALF1, UNC79 and UNC80 (PubMed:32494638, PubMed:33203861). NALCN is the voltage-sensing, pore-forming subunit of the NALCN channel complex (PubMed:17448995). NALCN channel complex is constitutively active and conducts monovalent cations but is blocked by physiological concentrations of extracellular divalent cations (PubMed:32494638). In addition to its role in regulating neuronal excitability, is required for normal respiratory rhythm, systemic osmoregulation by controlling the serum sodium concentration and in the regulation of the intestinal pace-making activity in the interstitial cells of Cajal (By similarity). NALCN channel is also activated by neuropeptides such as neurotensin and substance P (SP) through a SRC family kinases-dependent pathway (By similarity). In addition, NALCN activity is enhanced/modulated by several GPCRs, such as CHRM3 (By similarity).

Targets

Nalcn, Nalcn

Alternative names

VGCNL1, Sodium leak channel NALCN, CanIon, Sodium leak channel non-selective protein, Voltage gated channel-like protein 1

Rabbit Recombinant Monoclonal NALCN antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29666-91

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling VGCNL1 with ab323186 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining staining on mouse lung.

    The section was incubated with ab323186 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling VGCNL1 with ab323186 at 1/1000 (0.507 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human lung (PMID: 10094463).

    The section was incubated with ab323186 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Immunohistochemical analysis of paraffin-embedded Human thalamus tissue labeling VGCNL1 with ab323186 at 1/1000 (0.507 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human thalamus (PMID: 10094463).

    The section was incubated with ab323186 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver, lung (PMID:10094463).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 50 µg

    Lane 2: Human thalamus tissue lysate at 50 µg

    Lane 3: Human hypothalamus tissue lysate at 50 µg

    Lane 4: Human lung tissue lysate at 50 µg

    Lane 5: Human liver tissue lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling VGCNL1 with ab323186 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on rat lung (PMID: 10094463).

    The section was incubated with ab323186 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VGCNL1 with ab323186 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebrum (PMID: 10094463).

    The section was incubated with ab323186 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VGCNL1 with ab323186 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining staining on mouse cerebrum.

    The section was incubated with ab323186 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver, lung (PMID:10094463).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186) at 1/1000 dilution

    Lane 1: Rat hippocampus tissue lysate at 50 µg

    Lane 2: Rat brain tissue lysate at 50 µg

    Lane 3: Rat hypothalamus tissue lysate at 50 µg

    Lane 4: Rat liver tissue lysate at 50 µg

    Lane 5: Rat lung tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 6s

  • Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186), expandable thumbnail

    Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver, lung (PMID:10094463).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-VGCNL1 antibody [EPR29666-91] (ab323186) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 50 µg

    Lane 2: Mouse thalamus tissue lysate at 50 µg

    Lane 3: Mouse brain tissue lysate at 50 µg

    Lane 4: Mouse liver tissue lysate at 50 µg

    Lane 5: Mouse lung tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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