AB272913

Anti-VGluT1 antibody

Name: Anti-VGluT1 antibody (ab272913) | Abcam
Brand: Abcam Limited
SKU: AB272913

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(3 Publications)

Rabbit Polyclonal VGluT1 antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Slc17a7.

View Alternative Names

BNPI, VGLUT1, SLC17A7, Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

5 Images

Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody (AB272913)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody (AB272913)

Frozen sections of adult mouse cerebellum tissue stained for VGluT1 using ab272913 at 1/250 dilution in immunohistochemical analysis.

Red : Beta tubulin.

Blue : DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (AB272913)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (AB272913)

Paraffin-embedded mouse cerebellum tissue stained for VGluT1 using ab272913 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (AB272913)

Paraffin-embedded rat brain tissue stained for VGluT1 using ab272913 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody (AB272913)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody (AB272913)

4% PFA-fixed DIV14 rat E18 primary cortical neurons cells stained for VGluT1 using ab272913 at 1/500 dilution in ICC/IF.

Red : Beta tubulin.

Blue : DAPI.

Supplier Data

Western blot - Anti-VGluT1 antibody (AB272913)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-VGluT1 antibody (ab272913) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IHC-Fr, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Slc17a7. The exact immunogen used to generate this antibody is proprietary information.

Q3TXX4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VGluT1 or Vesicular Glutamate Transporter 1 is an important protein for transporting glutamate in the brain. It plays an important role in packaging glutamate into synaptic vesicles. This protein has an approximate mass of 61 kDa. Scientists find VGluT1 expression mainly in the central nervous system especially in areas involved in synaptic transmission and neural communication. VGluT1 localizes at the presynaptic terminals and enables glutamate to be stored in vesicles before release into the synaptic cleft.

Biological function summary

The function of VGluT1 centers on neurotransmitter regulation maintaining adequate synaptic glutamate levels. It does not work alone but works as part of a complex within the presynaptic vesicular organelles. This transport system enables efficient glutamate signaling essential for learning and memory. Through its action it supports important processes in brain development and synaptic plasticity indicating its role in cognitive functions.

Pathways

VGluT1 is essential in the glutamatergic signaling pathways which influence numerous neurotransmission activities. Its activity interlinks with proteins like synaptophysin and synapsins that help dock and release synaptic vesicles. Another associated pathway involves glutamate synthesis and recycling helping maintain excitatory neurotransmission balance. These pathways implicate VGluT1 in maintaining proper neural network connectivity and excitatory-inhibitory balance.

Altered VGluT1 function impacts conditions such as schizophrenia and amyotrophic lateral sclerosis (ALS). Researchers associate its dysregulation with impaired glutamate homeostasis leading to synaptic dysfunction. VGluT1 interacts with neuronal proteins such as EAAT2 involved in ALS where its malfunction results in motor neuron degeneration. In the context of schizophrenia altered VGluT1 expression disturbs glutamatergic neurotransmission contributing to symptoms due to synaptic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 10820226). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (By similarity). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (By similarity). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular K(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed : 10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (By similarity). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity).

See full target information SLC17A7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biology 14: PubMed41007382

2025

A Simple Three-Dimensional Compartmentalized Co-Culture Model for Basal Forebrain and Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Luo,Jing Li,Zhiyu Deng,Yali Xu,Xixi Li,Miao Ren,Xiangning Li

Frontiers in oncology 15:1508819 PubMed40391158

2025

Repurposing riluzole as an anti-osteosarcoma agent.

Applications

Unspecified application

Species

Unspecified reactive species

Okkeun Jung,Vinagolu K Rajasekhar,Syeda Maryam Azeem,Shraddha ChandThakuri,Beatrice Norton,John H Healey,Shahana Mahajan

Communications biology 7:177 PubMed38418614

2024

Modular tissue-in-a-CUBE platform to model blood-brain barrier (BBB) and brain interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Koh,Masaya Hagiwara

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VGluT1 antibody

Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody (AB272913)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (AB272913)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (AB272913)

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody (AB272913)

Western blot - Anti-VGluT1 antibody (AB272913)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise