AB240224

Anti-VGluT1 antibody [EPR10953] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal VGluT1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

BNPI, VGLUT1, SLC17A7, Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR10953] - BSA and Azide free (AB240224)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR10953] - BSA and Azide free (AB240224)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling VGluT1 with ab180188 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180188).

Supplier Data

Western blot - Anti-VGluT1 antibody [EPR10953] - BSA and Azide free (AB240224)

This data was developed using the same antibody clone in a different buffer formulation containing sodium citrate, sodium chloride, EDTA, PBS, Glycerol and BSA (ab180188).

All lanes:

Western blot - Anti-VGluT1 antibody [EPR10953] - C-terminal (<a href='/en-us/products/primary-antibodies/vglut1-antibody-epr10953-c-terminal-ab180188'>ab180188</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Neuro-2a (Mouse neuroblastoma cell line) lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cell line) lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

Unconjugated

Anti-VGluT1 antibody [EPR10953] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-VGluT1 antibody [EPR10953] – C–terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-VGluT1 antibody [EPR10953] – C–terminal
578 PE

PE Anti-VGluT1 antibody [EPR10953] – C–terminal
660 APC

APC Anti-VGluT1 antibody [EPR10953] – C–terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-VGluT1 antibody [EPR10953] – C–terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-VGluT1 antibody [EPR10953] – C–terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10953

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240224 is the carrier-free version of ab180188.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VGluT1 or Vesicular Glutamate Transporter 1 is an important protein for transporting glutamate in the brain. It plays an important role in packaging glutamate into synaptic vesicles. This protein has an approximate mass of 61 kDa. Scientists find VGluT1 expression mainly in the central nervous system especially in areas involved in synaptic transmission and neural communication. VGluT1 localizes at the presynaptic terminals and enables glutamate to be stored in vesicles before release into the synaptic cleft.

Biological function summary

The function of VGluT1 centers on neurotransmitter regulation maintaining adequate synaptic glutamate levels. It does not work alone but works as part of a complex within the presynaptic vesicular organelles. This transport system enables efficient glutamate signaling essential for learning and memory. Through its action it supports important processes in brain development and synaptic plasticity indicating its role in cognitive functions.

Pathways

VGluT1 is essential in the glutamatergic signaling pathways which influence numerous neurotransmission activities. Its activity interlinks with proteins like synaptophysin and synapsins that help dock and release synaptic vesicles. Another associated pathway involves glutamate synthesis and recycling helping maintain excitatory neurotransmission balance. These pathways implicate VGluT1 in maintaining proper neural network connectivity and excitatory-inhibitory balance.

Altered VGluT1 function impacts conditions such as schizophrenia and amyotrophic lateral sclerosis (ALS). Researchers associate its dysregulation with impaired glutamate homeostasis leading to synaptic dysfunction. VGluT1 interacts with neuronal proteins such as EAAT2 involved in ALS where its malfunction results in motor neuron degeneration. In the context of schizophrenia altered VGluT1 expression disturbs glutamatergic neurotransmission contributing to symptoms due to synaptic imbalance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 10820226). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (By similarity). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (By similarity). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular K(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed : 10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (By similarity). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity).

See full target information SLC17A7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:115008 PubMed39666457

2024

The nucleoporin Nup153 is the anchor for Kif1a during basal nuclear migration in brain progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Falnikar,Sebastian Quintremil,Hung-Jun Zhao,Haw-Yuan Cheng,Paige Helmer,Jin-Wu Tsai,Richard B Vallee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VGluT1 antibody [EPR10953] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR10953] - BSA and Azide free (AB240224)

Western blot - Anti-VGluT1 antibody [EPR10953] - BSA and Azide free (AB240224)

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Publications (1)

Product promise