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Anti-VGluT1 antibody [EPR22269] ab227805 is a rabbit monoclonal antibody that is used in VGluT1 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests


Images

Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (AB227805), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (AB227805), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR22269] (AB227805), expandable thumbnail
  • Western blot - Anti-VGluT1 antibody [EPR22269] (AB227805), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (AB227805), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 49% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-FrIHC-P
Human
Expected
Expected
Tested
Tested
Tested
Mouse
Not recommended
Tested
Tested
Tested
Tested
Rat
Tested
Expected
Tested
Tested
Tested

Tested
Tested

Species

Rat

Dilution info

1 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.1 µg/mL

Notes

-

Species

Rat

Dilution info

0.1 µg/mL

Notes

-

Species

Human

Dilution info

0.1 µg/mL

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Alternative names

Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7, SLC17A7, BNPI, VGLUT1

Recommended products

Anti-VGluT1 antibody [EPR22269] ab227805 is a rabbit monoclonal antibody that is used in VGluT1 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22269

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VGluT1 or Vesicular Glutamate Transporter 1 is an important protein for transporting glutamate in the brain. It plays an important role in packaging glutamate into synaptic vesicles. This protein has an approximate mass of 61 kDa. Scientists find VGluT1 expression mainly in the central nervous system especially in areas involved in synaptic transmission and neural communication. VGluT1 localizes at the presynaptic terminals and enables glutamate to be stored in vesicles before release into the synaptic cleft.

Biological function summary

The function of VGluT1 centers on neurotransmitter regulation maintaining adequate synaptic glutamate levels. It does not work alone but works as part of a complex within the presynaptic vesicular organelles. This transport system enables efficient glutamate signaling essential for learning and memory. Through its action it supports important processes in brain development and synaptic plasticity indicating its role in cognitive functions.

Pathways

VGluT1 is essential in the glutamatergic signaling pathways which influence numerous neurotransmission activities. Its activity interlinks with proteins like synaptophysin and synapsins that help dock and release synaptic vesicles. Another associated pathway involves glutamate synthesis and recycling helping maintain excitatory neurotransmission balance. These pathways implicate VGluT1 in maintaining proper neural network connectivity and excitatory-inhibitory balance.

Associated diseases and disorders

Altered VGluT1 function impacts conditions such as schizophrenia and amyotrophic lateral sclerosis (ALS). Researchers associate its dysregulation with impaired glutamate homeostasis leading to synaptic dysfunction. VGluT1 interacts with neuronal proteins such as EAAT2 involved in ALS where its malfunction results in motor neuron degeneration. In the context of schizophrenia altered VGluT1 expression disturbs glutamatergic neurotransmission contributing to symptoms due to synaptic imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    IHC image of vGluT1 staining in a section of frozen normal human cerebellum performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab227805, 0.1ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    IHC image of vGluT1 staining in a section of frozen normal human cerebellum performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab227805, 0.1ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR22269] (ab227805)

    ab227805 staining VGluT1 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab227805 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Different lysate preparation and boiling methods resulted in different banding patterns observed. The lysates in lanes 1 and 3 were prepared by RIPA lysis method with and without boiling. The lysate in lane 2 was prepared by 1% SDS Hot lysis method. For WB sample preparation, we recommend using RIPA lysis buffer and without boiling.

    All lanes: Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805) at 1/1000 dilution

    All lanes: Mouse brain lysate at 20 µg/mL

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 2 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 62 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling VGluT1 with ab227805 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human cerebral cortex (PMID: 29532891). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunoprecipitation - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunoprecipitation - Anti-VGluT1 antibody [EPR22269] (ab227805)

    VGluT1 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab227805 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227805 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 μg (Input).

    Lane 2: ab227805 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab227805 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    The pattern of oligomeric/dimeric forms observed is consistent with what has been described in the literature (PMID: 15192755).

    All lanes: Immunoprecipitation - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling VGluT1 with ab227805 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebral cortex (PMID: 29532891) is observed. Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VGluT1 with ab227805 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Specific cytoplasmic staining on mouse hippocampus, positive staining was also observed on mouse cerebral cortex and thalamus (PMID: 29532891). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling VGluT1 with ab227805 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on rat hippocampus (PMID: 29532891) is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling VGluT1 with ab227805 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse hippocampus (PMID: 29532891) is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Oligomeric forms observed in the human brain lysate were due to the 1% SDS Hot lysate preparation method, consistent with what has been described in the literature (PMID: 15192755).

    All lanes: Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805) at 1/1000 dilution

    All lanes: Human brain lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 62 kDa

    Exposure time: 3min

  • Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Brain lysates were prepared with RIPA lysis buffer. The pattern of oligomers/dimers observed is consistent with what has been described in the literature (PMID: 15192755).

    All lanes: Western blot - Anti-VGluT1 antibody [EPR22269] (ab227805) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Rat brain lysate at 20 µg

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 62 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VGluT1 with ab227805 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: No staining on human liver is observed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling VGluT1 with ab227805 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: No staining on rat liver is observed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [EPR22269] (ab227805)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VGluT1 with ab227805 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: No staining on mouse liver.

  • Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR22269] (ab227805), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR22269] (ab227805)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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