Anti-VGluT1 antibody [N28-9] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [N28-9] - BSA and Azide free (AB264108)

IHC image of VGluT1 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134283, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab134283).

• WB

Project

Western blot - Anti-VGluT1 antibody [N28-9] - BSA and Azide free (AB264108)

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134283 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134283).

All lanes:

Western blot - Anti-VGluT1 antibody [N28-9] (<a href='/en-us/products/primary-antibodies/vglut1-antibody-n28-9-ab134283'>ab134283</a>) at 5 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 20 µg

Lane 2:

Brain (Rat) Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

Predicted band size: 61 kDa

Observed band size: 50 kDa,55 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - Anti-VGluT1 antibody [N28-9] - BSA and Azide free (AB264108)

All lanes:

Western blot - Anti-VGluT1 antibody [N28-9] (<a href='/en-us/products/primary-antibodies/vglut1-antibody-n28-9-ab134283'>ab134283</a>) at 5 µg

Lane 1:

Human Brain Tissue Lysate at 20 µg

Lane 2:

Rat Brain Tissue Lysate at 20 µg

Lane 3:

Mouse Brain Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

Predicted band size: 61 kDa

Observed band size: 200 kDa,50 kDa,55 kDa

true

Exposure time: 30s