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AB305253

Anti-VGLUT2 antibody [EPR25147-68]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal VGLUT2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DNPI, VGLUT2, SLC17A6, Vesicular glutamate transporter 2, VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] (AB305253)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] (AB305253)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling VGLUT2 with ab305253 at 1/100 (4.57 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : no staining on human lung. The section was incubated with ab305253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] (AB305253)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] (AB305253)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling VGLUT2 with ab305253 at 1/100 (4.57 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebellum. The section was incubated with ab305253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] (AB305253)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] (AB305253)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling VGLUT2 with ab305253 at 1/100 (4.57 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab305253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Carrier free

    Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25147-68

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VGLUT2 also known as Vesicular Glutamate Transporter 2 or SLC17A6 plays a significant role in neurotransmission. This protein facilitates the transport of glutamate an excitatory neurotransmitter into synaptic vesicles in preparation for synaptic release. VGLUT2 has a molecular weight of approximately 61 kDa. Its expression appears particularly in the central nervous system including regions like the thalamus brainstem and some parts of the cerebral cortex.
Biological function summary

VGLUT2 is essential for proper glutamatergic signaling in neuronal communication. The protein does not form part of a larger complex but functions individually to maintain synaptic vesicle filling with glutamate. Normal glutamatergic synaptic transmission relies on VGLUT2’s ability to concentrate glutamate in synaptic vesicles. This concentration is necessary for effective excitatory signaling impacting processes such as synaptic plasticity learning and memory.

Pathways

VGLUT2 serves a critical function in neurotransmitter release and synaptic vesicle cycle. The protein actively transports glutamate into synaptic vesicles enabling its release into the synaptic cleft. VGLUT2 interacts closely with proteins like SNAREs that assist in vesicle docking and fusion. The protein also plays a functional role in the glutamatergic pathway which is important for excitatory neurotransmission in the brain.

VGLUT2 holds relevance to conditions such as schizophrenia and epilepsy. Its dysregulation can alter glutamate levels potentially contributing to altered neuronal excitability and synaptic dysfunction. Studies link VGLUT2 with connected proteins like NMDA receptors which mediate excitatory postsynaptic potentials and are associated with glutamatergic signaling disruptions in these disorders. Alterations in the expression or function of VGLUT2 might influence susceptibility to these neuropsychiatric and neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 11698620, PubMed : 33440152). At the synaptic vesicle membrane, mainly functions as a uniporter which transports preferentially L-glutamate but also, phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed : 11698620). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed : 11698620). In addition, functions as a chloride channel that allows the chloride permeation through the synaptic vesicle membrane therefore affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, functions as a vesicular K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular H(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (Probable) (PubMed : 10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (Probable). Also involved in the regulation of retinal hyaloid vessel regression during postnatal development (By similarity). May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas (By similarity).
See full target information SLC17A6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2083-2094 PubMed40335722

2025

Identification of putative baroreceptors in human aortic arch by histological and omics analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Yankey Yundung,Jaroslav Pelisek,Umberto Maccio,Zsuzsanna Varga,Houria Ech-Cherif,Enni Markkanen,Fabian Rössler,Ahmed Ouda,Omer Dzemali,Alexander Zimmermann,Benedikt Reutersberg
View all publications
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Product promise

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