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AB305254

Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free

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Rabbit Recombinant Monoclonal VGLUT2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

DNPI, VGLUT2, SLC17A6, Vesicular glutamate transporter 2, VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free (AB305254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free (AB305254)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free (AB305254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free (AB305254)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free (AB305254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGLUT2 antibody [EPR25147-68] - BSA and Azide free (AB305254)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25147-68

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VGLUT2 also known as Vesicular Glutamate Transporter 2 or SLC17A6 plays a significant role in neurotransmission. This protein facilitates the transport of glutamate an excitatory neurotransmitter into synaptic vesicles in preparation for synaptic release. VGLUT2 has a molecular weight of approximately 61 kDa. Its expression appears particularly in the central nervous system including regions like the thalamus brainstem and some parts of the cerebral cortex.
Biological function summary

VGLUT2 is essential for proper glutamatergic signaling in neuronal communication. The protein does not form part of a larger complex but functions individually to maintain synaptic vesicle filling with glutamate. Normal glutamatergic synaptic transmission relies on VGLUT2’s ability to concentrate glutamate in synaptic vesicles. This concentration is necessary for effective excitatory signaling impacting processes such as synaptic plasticity learning and memory.

Pathways

VGLUT2 serves a critical function in neurotransmitter release and synaptic vesicle cycle. The protein actively transports glutamate into synaptic vesicles enabling its release into the synaptic cleft. VGLUT2 interacts closely with proteins like SNAREs that assist in vesicle docking and fusion. The protein also plays a functional role in the glutamatergic pathway which is important for excitatory neurotransmission in the brain.

VGLUT2 holds relevance to conditions such as schizophrenia and epilepsy. Its dysregulation can alter glutamate levels potentially contributing to altered neuronal excitability and synaptic dysfunction. Studies link VGLUT2 with connected proteins like NMDA receptors which mediate excitatory postsynaptic potentials and are associated with glutamatergic signaling disruptions in these disorders. Alterations in the expression or function of VGLUT2 might influence susceptibility to these neuropsychiatric and neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 11698620, PubMed : 33440152). At the synaptic vesicle membrane, mainly functions as a uniporter which transports preferentially L-glutamate but also, phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed : 11698620). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed : 11698620). In addition, functions as a chloride channel that allows the chloride permeation through the synaptic vesicle membrane therefore affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, functions as a vesicular K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular H(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (Probable) (PubMed : 10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (Probable). Also involved in the regulation of retinal hyaloid vessel regression during postnatal development (By similarity). May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas (By similarity).
See full target information SLC17A6
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Product promise

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