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AB316206

Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free

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Rabbit Recombinant Monoclonal VGLUT3 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Mouse, Mouse samples.

View Alternative Names

Vglut3, Gerp, Rnf27, Trim8, Vesicular glutamate transporter 3, VGluT3, Solute carrier family 17 member 8

3 Images
Western blot - Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free (AB316206)
  • WB

Supplier Data

Western blot - Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free (AB316206)

This data was developed using ab316205, the same antibody clone in a different buffer formulation.

The identity of the bands between 37 kDa and 50 kDa are unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-VGLUT3 antibody [EPR28534-131] (<a href='/en-us/products/primary-antibodies/vglut3-antibody-epr28534-131-ab316205'>ab316205</a>) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 50 µg

Lane 2:

Mouse brain tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 66 kDa,36 kDa

true

Exposure time: 70s

Western blot - Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free (AB316206)
  • WB

Supplier Data

Western blot - Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free (AB316206)

This data was developed using ab316205, the same antibody clone in a different buffer formulation.

Low expression : cerebellum, spleen (PMID : 12097496).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-VGLUT3 antibody [EPR28534-131] (<a href='/en-us/products/primary-antibodies/vglut3-antibody-epr28534-131-ab316205'>ab316205</a>) at 1/1000 dilution

Lane 1:

Mouse striatum tissue lysate at 40 µg

Lane 2:

Mouse cerebellum tissue lysate at 40 µg

Lane 3:

Mouse spleen tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 66 kDa,124 kDa

true

Exposure time: 180s

Western blot - Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free (AB316206)
  • WB

Supplier Data

Western blot - Anti-VGLUT3 antibody [EPR28534-131] - BSA and Azide free (AB316206)

This data was developed using ab316205, the same antibody clone in a different buffer formulation.

This antibody does not cross-react with mouse VGLUT1 and VGLUT2.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-VGLUT3 antibody [EPR28534-131] (<a href='/en-us/products/primary-antibodies/vglut3-antibody-epr28534-131-ab316205'>ab316205</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse VGLUT3 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse VGLUT1 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse VGLUT2 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 66 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28534-131

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316206 is the carrirer-free version of ab316205.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VGLUT3 also known as Vesicular Glutamate Transporter 3 or SLC17A8 functions as a transporter responsible for loading the neurotransmitter glutamate into synaptic vesicles in neurons. It has a molecular mass of approximately 65 kDa. VGLUT3 expression occurs in specific regions of the central nervous system such as the hippocampus and striatum as well as in sensory regions like the cochlea. This transporter lacks the abundance compared to other VGLUTs making its presence more strategically targeted in its roles.
Biological function summary

VGLUT3 modulates neurotransmitter signaling by facilitating glutamate release in both excitatory and modulatory circuits. It operates not strictly as part of a homomeric complex but interacts closely with other synaptic proteins to regulate neurotransmission. VGLUT3 presence in serotonin and dopamine neurons indicates its role in modulating their transmission suggesting a diverse function beyond typical excitatory neurotransmitter release. This transporter's activity influences synaptic plasticity and firing patterns vital for maintaining neural circuit balance.

Pathways

VGLUT3 integrates into the glutamatergic transmission pathway and the modulation of dopaminergic and serotonergic pathways. It works alongside VGLUT1 and VGLUT2 though each has a different regional brain expression allowing diverse neurotransmitter interactions in neural signaling. The transporter interacts with synaptic machinery influencing pathways responsible for synaptic vesicle recycling and neurotransmitter homeostasis ensuring effective transmission functions in responsive neurons.

VGLUT3 influences conditions such as epilepsy and schizophrenia. Its dysfunction can lead to improper glutamate levels in the synaptic cleft disrupting neural excitability and causing conditions like seizures or altered neuropsychiatric states. VGLUT3 shares connections with transport proteins like SLC1A1 affecting glutamate transport across membranes. Understanding these associations aids in therapeutic target development where modulating VGLUT3 function could address glutamatergic dysregulation contributing to these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, sodium and phosphate (PubMed : 12384506, PubMed : 18080752, PubMed : 18215623). At the synaptic vesicle membrane, mainly functions as an uniporter that mediates the uptake of L-glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed : 12384506, PubMed : 18080752, PubMed : 18215623). The L-glutamate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed : 12384506). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (By similarity). The symporter activity is electrogenic (By similarity). Moreover, operates synergistically with SLC18A3/VACHT under a constant H(+) gradient, thereby allowing striatal vesicular acetylcholine uptake (PubMed : 18278042).
See full target information Slc17a8
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Product promise

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