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AB315190

Anti-VILIP1 antibody [3A9]

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Mouse Monoclonal VILIP1 antibody. Suitable for WB, IHC-FoFr and reacts with Rat, Pig, Cow, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VSNL1.

View Alternative Names

VISL1, VSNL1, Visinin-like protein 1, VILIP, VLP-1, Hippocalcin-like protein 3, HLP3

2 Images
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-VILIP1 antibody [3A9] (AB315190)
  • IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-VILIP1 antibody [3A9] (AB315190)

Confocal image of adult rat cerebellum stained with ab315190, dilution 1 : 1,000, in green, and costained with chicken pAb to MAP2, CPCA-MAP2, dilution 1 : 10,000, in red. The blue is DAPI staining of nuclear DNA. The ab315190 antibody reveals perikarya and synaptic regions in the neuron rich granular layer (bottom) and synapse rich molecular layer (top). Note that the large prominent Purkinje neurons at the junction of these two layers do not stain with the ab315190 antibody, in line with the findings of others (4).

Western blot - Anti-VILIP1 antibody [3A9] (AB315190)
  • WB

Supplier Data

Western blot - Anti-VILIP1 antibody [3A9] (AB315190)

Western blot analysis of different tissue lysates using ab315190, dilution 1 : 1,000, in green : [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at about 20kDa corresponds to the VLP1 protein.

All lanes:

Western blot - Anti-VILIP1 antibody [3A9] (ab315190) at 1/1000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Mouse brain tissue lysate

Lane 4:

Pig hippocampus tissue lysate

Lane 5:

Cow cerebellum tissue lysate

Observed band size: 20 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A9

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Pig, Cow

Applications

WB, IHC-FoFr

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VSNL1.

P62760

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Proteins & Peptides

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Primary Antibodies

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  • 5
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Reactivity data

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Visinin-like protein 1 commonly abbreviated as VILIP1 is a neuronal calcium sensor protein. It has a mass of about 22 kDa and consists of four EF-hand motifs that bind calcium ions. VILIP1 is primarily expressed in the central nervous system including regions such as the hippocampus and cerebral cortex. This protein interacts with membranes and other intracellular molecular targets in response to changes in calcium levels influencing cellular activities associated with calcium signaling.
Biological function summary

Visinin-like protein 1 modulates various physiological processes within neurons. It regulates signal transduction pathways by mediating calcium-dependent interactions with other proteins. VILIP1 may be part of complexes with other signaling proteins that participate in neurotransmitter release and synaptic plasticity. Its ability to modulate the phosphorylation status of its binding partners can influence neuronal excitability and synaptic communication.

Pathways

Various studies highlight how visinin-like protein 1 participates in critical signaling modules. This protein contributes to the regulation of the cAMP signaling pathway where it can affect the activation of adenylate cyclase. Moreover VILIP1 interacts with signaling pathways related to nicotinic acetylcholine receptors affecting the transmission of nerve impulses. It influences the dynamics between calcium signaling and cAMP affecting processes like learning and memory.

Visinin-like protein 1 relates significantly to neurodegenerative diseases. Research findings associate elevated VILIP1 levels with Alzheimer's disease where it can serve as a biomarker for disease progression. Furthermore it connects to Parkinson's disease given its role in neuronal calcium homeostasis a critical factor in disease pathology. In Alzheimer's disease VILIP1 levels can correlate with beta-amyloid plaque development and tau protein phosphorylation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
See full target information VSNL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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