Rabbit Recombinant Monoclonal VILIP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-VILIP1 antibody [EPR9833] (AB151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--western-blot-img57283.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VILIP1 antibody [EPR9833] (AB151741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135313.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134920.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134573.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131886.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (AB151741)")
Publications
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- The Journal of steroid biochemistry and molecular biology 193:1054222019DLK1/PREF1 marks a novel cell population in the human adrenal cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Hadjidemetriou,Katia Mariniello,Gerard Ruiz-Babot,James Pittaway,Alessandra Mancini,Demetris Mariannis,Celso E Gomez-Sanchez,Laila Parvanta,William M Drake,Teng-Teng Chung,Tarek Ezzat Abdel-Aziz,Aimee DiMarco,Fausto F Palazzo,Margaret E Wierman,Katja Kiseljak-Vassiliades,Peter J King,Leonardo GuastiPubMed 31265901
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Not recommended | Expected | Not recommended |
| Rat | Predicted | Not recommended | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
Alternative names
VISL1, VSNL1, Visinin-like protein 1, VILIP, VLP-1, Hippocalcin-like protein 3, HLP3
Recommended products
Rabbit Recombinant Monoclonal VILIP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9833
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Visinin-like protein 1 commonly abbreviated as VILIP1 is a neuronal calcium sensor protein. It has a mass of about 22 kDa and consists of four EF-hand motifs that bind calcium ions. VILIP1 is primarily expressed in the central nervous system including regions such as the hippocampus and cerebral cortex. This protein interacts with membranes and other intracellular molecular targets in response to changes in calcium levels influencing cellular activities associated with calcium signaling.
- Biological function summary
Visinin-like protein 1 modulates various physiological processes within neurons. It regulates signal transduction pathways by mediating calcium-dependent interactions with other proteins. VILIP1 may be part of complexes with other signaling proteins that participate in neurotransmitter release and synaptic plasticity. Its ability to modulate the phosphorylation status of its binding partners can influence neuronal excitability and synaptic communication.
- Pathways
Various studies highlight how visinin-like protein 1 participates in critical signaling modules. This protein contributes to the regulation of the cAMP signaling pathway where it can affect the activation of adenylate cyclase. Moreover VILIP1 interacts with signaling pathways related to nicotinic acetylcholine receptors affecting the transmission of nerve impulses. It influences the dynamics between calcium signaling and cAMP affecting processes like learning and memory.
- Associated diseases and disorders
Visinin-like protein 1 relates significantly to neurodegenerative diseases. Research findings associate elevated VILIP1 levels with Alzheimer's disease where it can serve as a biomarker for disease progression. Furthermore it connects to Parkinson's disease given its role in neuronal calcium homeostasis a critical factor in disease pathology. In Alzheimer's disease VILIP1 levels can correlate with beta-amyloid plaque development and tau protein phosphorylation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--western-blot-img57283.jpg "Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741)
All lanes: Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human cerebellum lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135313.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab151741 at 1/100 diluton.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134920.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling VILIP1 with ab151741 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134573.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
Immunohistochemical analysis of paraffin embedded Human Retina tissue using ab151741 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131886.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151741 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136048.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab151741 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82445.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151741 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136048.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741), expandable thumbnail](https://content.abcam.com/products/images/vilip1-antibody-epr9833-ab151741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82445.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)")
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