AB188106

Anti-VILIP1 antibody [EPR9833] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal VILIP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

VISL1, VSNL1, Visinin-like protein 1, VILIP, VLP-1, Hippocalcin-like protein 3, HLP3

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151741 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab151741 at 1/100 diluton.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling VILIP1 with ab151741 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Retina tissue using ab151741 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151741 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab151741 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

This data was developed using ab151741, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VILIP1 antibody [EPR9833] (<a href='/en-us/products/primary-antibodies/vilip1-antibody-epr9833-ab151741'>ab151741</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 22 kDa

false

Unconjugated

Anti-VILIP1 antibody [EPR9833]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9833

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab188106 is the carrier-free version of ab151741.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Visinin-like protein 1 commonly abbreviated as VILIP1 is a neuronal calcium sensor protein. It has a mass of about 22 kDa and consists of four EF-hand motifs that bind calcium ions. VILIP1 is primarily expressed in the central nervous system including regions such as the hippocampus and cerebral cortex. This protein interacts with membranes and other intracellular molecular targets in response to changes in calcium levels influencing cellular activities associated with calcium signaling.

Biological function summary

Visinin-like protein 1 modulates various physiological processes within neurons. It regulates signal transduction pathways by mediating calcium-dependent interactions with other proteins. VILIP1 may be part of complexes with other signaling proteins that participate in neurotransmitter release and synaptic plasticity. Its ability to modulate the phosphorylation status of its binding partners can influence neuronal excitability and synaptic communication.

Pathways

Various studies highlight how visinin-like protein 1 participates in critical signaling modules. This protein contributes to the regulation of the cAMP signaling pathway where it can affect the activation of adenylate cyclase. Moreover VILIP1 interacts with signaling pathways related to nicotinic acetylcholine receptors affecting the transmission of nerve impulses. It influences the dynamics between calcium signaling and cAMP affecting processes like learning and memory.

Visinin-like protein 1 relates significantly to neurodegenerative diseases. Research findings associate elevated VILIP1 levels with Alzheimer's disease where it can serve as a biomarker for disease progression. Furthermore it connects to Parkinson's disease given its role in neuronal calcium homeostasis a critical factor in disease pathology. In Alzheimer's disease VILIP1 levels can correlate with beta-amyloid plaque development and tau protein phosphorylation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.

See full target information VSNL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed38791874

2024

Pepsinogen C Interacts with IQGAP1 to Inhibit the Metastasis of Gastric Cancer Cells by Suppressing Rho-GTPase Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxi Ding,Yingnan Liu,Xiaodong Lu,Aoran Liu,Qian Xu,Yuan Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VILIP1 antibody [EPR9833] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Western blot - Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (AB188106)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise