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AB201989

Anti-Villin antibody [3E5G11] - N-terminal

4

(5 Reviews)

|

(11 Publications)

Mouse Monoclonal Villin antibody. N-terminal. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 1-250.

View Alternative Names

VIL, VIL1, Villin-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Villin with ab201989 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

Immunofluorescence analysis of Hela cells using ab201989 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

Flow cytometric analysis of SW620 cells using ab201989 at 1/200 dilution (green) and negative control (red).

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

All lanes:

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution

All lanes:

Human Villin (AA: 1-209) recombinant protein

Predicted band size: 93 kDa

false

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

All lanes:

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Villin (AA: 1-209)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 93 kDa

false

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

All lanes:

Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution

All lanes:

SW620 cell lysate

Predicted band size: 93 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E5G11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P09327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR286745-12 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 4:100903 PubMed39561715

2024

Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor.

Applications

Unspecified application

Species

Unspecified reactive species

Shicheng Ye,Ary Marsee,Gilles S van Tienderen,Mohammad Rezaeimoghaddam,Hafsah Sheikh,Roos-Anne Samsom,Eelco J P de Koning,Sabine Fuchs,Monique M A Verstegen,Luc J W van der Laan,Frans van de Vosse,Jos Malda,Keita Ito,Bart Spee,Kerstin Schneeberger

Gut microbes 16:2313769 PubMed38353638

2024

Gut microbiota regulates host melatonin production through epithelial cell MyD88.

Applications

Unspecified application

Species

Unspecified reactive species

Bingnan Liu,Lijuan Fan,Youxia Wang,Hao Wang,Yuqi Yan,Shuai Chen,Ifen Hung,Chunxue Liu,Hong Wei,Liangpeng Ge,Wenkai Ren

Signal transduction and targeted therapy 7:392 PubMed36529763

2022

Analogous comparison unravels heightened antiviral defense and boosted viral infection upon immunosuppression in bat organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Cun Li,Zhixin Wan,Man Chun Chiu,Jingjing Huang,Yifei Yu,Lin Zhu,Jian-Piao Cai,Lei Rong,You-Qiang Song,Hin Chu,Zongwei Cai,Shibo Jiang,Kwok-Yung Yuen,Jie Zhou

Nature methods 19:1449-1460 PubMed36280722

2022

Geometric engineering of organoid culture for enhanced organogenesis in a dish.

Applications

Unspecified application

Species

Unspecified reactive species

Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh

Pharmaceutics 14: PubMed35893808

2022

Mimicking the Intestinal Host-Pathogen Interactions in a 3D In Vitro Model: The Role of the Mucus Layer.

Applications

Unspecified application

Species

Unspecified reactive species

María García-Díaz,Maria Del Mar Cendra,Raquel Alonso-Roman,María Urdániz,Eduard Torrents,Elena Martínez

Genome biology 23:106 PubMed35484598

2022

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Yunuo Mao,Wendong Wang,Xin Zhou,Wei Wang,Shuai Gao,Jingyun Li,Lu Wen,Wei Fu,Fuchou Tang

Tissue engineering and regenerative medicine 19:403-415 PubMed35122584

2022

Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui Yang

Scientific reports 10:8877 PubMed32483224

2020

Mouse intestinal tuft cells express advillin but not villin.

Applications

Unspecified application

Species

Unspecified reactive species

Amin Esmaeilniakooshkghazi,Sudeep P George,Ritwika Biswas,Seema Khurana

Frontiers in bioengineering and biotechnology 8:294 PubMed32318564

2020

Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Altay,Sébastien Tosi,María García-Díaz,Elena Martínez

Toxins 11: PubMed31590302

2019

Mycotoxin Occurrence in Maize Silage-A Neglected Risk for Bovine Gut Health?

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Reisinger,Sonja Schürer-Waldheim,Elisabeth Mayer,Sandra Debevere,Gunther Antonissen,Michael Sulyok,Veronika Nagl
View all publications
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