Mouse Monoclonal Villin antibody. N-terminal. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 1-250.
Images
![Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (AB201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--flow-cytometry-img121406.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (AB201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126365.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)")
![Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (AB201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--immunocytochemistry-immunofluorescence-img5067.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)")
![Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--western-blot-img65073.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)")
![Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--western-blot-img156527.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)")
Publications
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- Signal transduction and targeted therapy 7:3922022Analogous comparison unravels heightened antiviral defense and boosted viral infection upon immunosuppression in bat organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojuan Liu,Cun Li,Zhixin Wan,Man Chun Chiu,Jingjing Huang,Yifei Yu,Lin Zhu,Jian-Piao Cai,Lei Rong,You-Qiang Song,Hin Chu,Zongwei Cai,Shibo Jiang,Kwok-Yung Yuen,Jie ZhouPubMed 36529763
- Pharmaceutics 14:2022Mimicking the Intestinal Host-Pathogen Interactions in a 3D In Vitro Model: The Role of the Mucus Layer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría García-Díaz,Maria Del Mar Cendra,Raquel Alonso-Roman,María Urdániz,Eduard Torrents,Elena MartínezPubMed 35893808
- Genome biology 23:1062022Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Wang,Yunuo Mao,Wendong Wang,Xin Zhou,Wei Wang,Shuai Gao,Jingyun Li,Lu Wen,Wei Fu,Fuchou TangPubMed 35484598
- Tissue engineering and regenerative medicine 19:403-4152022Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui YangPubMed 35122584
- Scientific reports 10:88772020Mouse intestinal tuft cells express advillin but not villin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmin Esmaeilniakooshkghazi et. al.PubMed 32483224
- Frontiers in bioengineering and biotechnology 8:2942020Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGizem Altay et. al.PubMed 32318564
- Toxins 11:2019Mycotoxin Occurrence in Maize Silage-A Neglected Risk for Bovine Gut Health?Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole Reisinger et. al.PubMed 31590302
- Cell host & microbe 26:123-134.e82019A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgia Wilke et. al.PubMed 31231046
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human VIL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P09327
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
Alternative names
VIL, VIL1, Villin-1
Recommended products
Mouse Monoclonal Villin antibody. N-terminal. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 1-250.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human VIL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P09327
- Clone number
- 3E5G11
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to supernatant. Lot no's high than GR286745-12 are from Tissue Culture Supernatant
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
![Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--flow-cytometry-img121406.jpg "Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
Flow cytometric analysis of SW620 cells using ab201989 at 1/200 dilution (green) and negative control (red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126365.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Villin with ab201989 at 1/200 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--immunocytochemistry-immunofluorescence-img5067.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
Immunofluorescence analysis of Hela cells using ab201989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
![Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--western-blot-img65073.jpg "Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
All lanes: Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution
All lanes: SW620 cell lysate
Predicted band size: 93 kDa
![Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--western-blot-img156527.jpg "Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
All lanes: Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution
Lane 1: HEK293 cell lysate
Lane 2: Villin (AA: 1-209)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 93 kDa
![Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--western-blot-img158828.jpg "Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
All lanes: Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution
All lanes: Human Villin (AA: 1-209) recombinant protein
Predicted band size: 93 kDa
![Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989), expandable thumbnail](https://content.abcam.com/products/images/villin-antibody-3e5g11-n-terminal-ab201989--western-blot-img158828.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)")
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