AB202021

Anti-Villin antibody [5E3B2]

Name: Anti-Villin antibody [5E3B2] (ab202021) | Abcam
Brand: Abcam Limited
SKU: AB202021

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(1 Publication)

Mouse Monoclonal Villin antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 1-250.

View Alternative Names

VIL, VIL1, Villin-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (AB202021)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (AB202021)

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue labeling Villin using ab202021 at 1/200 dilution, followed by DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (AB202021)

Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling Villin using ab202021 at 1/200 dilution, followed by DAB staining.

Supplier Data

Western blot - Anti-Villin antibody [5E3B2] (AB202021)

Expected MW is 49 kDa

All lanes:

Western blot - Anti-Villin antibody [5E3B2] (ab202021) at 1/500 dilution

All lanes:

Recombinant fragment of Villin (aa 1-209) at 1 µg

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-Villin antibody [5E3B2] (AB202021)

All lanes:

Western blot - Anti-Villin antibody [5E3B2] (ab202021) at 1/500 dilution

Lane 1:

Non transfected HEK293 cell lysate at 1 µg

Lane 2:

Villin (aa: 1-209)-hIgGFc transfected HEK293 cell lysate at 1 µg

Predicted band size: 93 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E3B2

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P09327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

See full target information VIL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:1825 PubMed35383176

2022

Structure-based design of stabilized recombinant influenza neuraminidase tetramers.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Ellis,Julia Lederhofer,Oliver J Acton,Yaroslav Tsybovsky,Sally Kephart,Christina Yap,Rebecca A Gillespie,Adrian Creanga,Audrey Olshefsky,Tyler Stephens,Deleah Pettie,Michael Murphy,Claire Sydeman,Maggie Ahlrichs,Sidney Chan,Andrew J Borst,Young-Jun Park,Kelly K Lee,Barney S Graham,David Veesler,Neil P King,Masaru Kanekiyo

View all publications

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Product promise

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