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AB233155

Anti-Villin antibody

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(2 Publications)

Rabbit Polyclonal Villin antibody. Suitable for WB, IHC-P and reacts with Pig, Mouse, Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 1-350.

View Alternative Names

VIL, VIL1, Villin-1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Villin with ab233155 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Villin with ab233155 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)

Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for Villin with ab233155 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Villin with ab233155 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)

Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for Villin with ab233155 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-Villin antibody (AB233155)
  • WB

Supplier Data

Western blot - Anti-Villin antibody (AB233155)

All lanes:

Western blot - Anti-Villin antibody (ab233155) at 3 µg/mL

Lane 1:

Mouse kidney tissue lysate

Lane 2:

Pig liver tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 93 kDa

false

Western blot - Anti-Villin antibody (AB233155)
  • WB

Supplier Data

Western blot - Anti-Villin antibody (AB233155)

All lanes:

Western blot - Anti-Villin antibody (ab233155) at 3 µg/mL

All lanes:

Recombinant human Villin protein

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P09327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1500105 PubMed39545066

2024

Epileptic seizures induced by pentylenetetrazole kindling accelerate Alzheimer-like neuropathology in 5×FAD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Zou,Chengyan Wang,Huang Li,Meihua Zhong,Jin Lin,Yan Hu,Zhou Chen,Chen-Ling Gan

Journal of proteomics 270:104739 PubMed36174954

2022

Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed Part II: Ileum proteomes.

Applications

Unspecified application

Species

Unspecified reactive species

Thi Xuan Nguyen,Alessandro Agazzi,Suzanne McGill,Stefan Weidt,Quang Hanh Han,Andrea Gelemanović,Mark McLaughlin,Giovanni Savoini,Peter David Eckersall,Richard Burchmore
View all publications
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Product promise

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