AB244291

Anti-Villin antibody

Name: Anti-Villin antibody (ab244291) | Abcam
Brand: Abcam Limited
SKU: AB244291
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Villin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 700 to C-terminus.

View Alternative Names

VIL, VIL1, Villin-1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human kidney tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human small intestine tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human liver tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human gallbladder tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human tonsil tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Negative control.

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB244291)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB244291)

PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for Villin (green) using ab244291 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human pancreas tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human colon tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Villin antibody (AB244291)

All lanes:

Western blot - Anti-Villin antibody (ab244291) at 0.4 µg/mL

Lane 1:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

See full target information VIL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1313 PubMed39900921

2025

USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Na Shao,Lei Xi,Yangfan Lv,Muhammad Idris,Lin Zhang,Ya Cao,Jingyi Xiang,Xi Xu,Belinda X Ong,Qiongyi Zhang,Xu Peng,Xiaoyan Yue,Feng Xu,Chungang Liu

View all publications

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Product promise

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