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AB272876

Anti-Villin antibody

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(1 Publication)

Rabbit Polyclonal Villin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1.

View Alternative Names

VIL, VIL1, Villin-1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB272876)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB272876)

Paraffin-embedded NCIN87 xenograft tissue stained for Villin using ab272876 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB272876)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB272876)

Methanol-fixed Hep3B (human hepatocellular carcinoma cell line) cells stained for Villin using ab272876 at 1/500 dilution in ICC/IF (green).

Co-stained with Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB272876)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB272876)

Immunohistochemical analysis of paraffin-embedded mouse intestine and duodenum tissue stained for Villin protein with ab272876 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Villin antibody (AB272876)
  • WB

Supplier Data

Western blot - Anti-Villin antibody (AB272876)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Villin antibody (ab272876) at 1/1000 dilution

All lanes:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 93 kDa

false

Western blot - Anti-Villin antibody (AB272876)
  • WB

Supplier Data

Western blot - Anti-Villin antibody (AB272876)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Villin antibody (ab272876) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 93 kDa

false

Western blot - Anti-Villin antibody (AB272876)
  • WB

Supplier Data

Western blot - Anti-Villin antibody (AB272876)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Villin antibody (ab272876) at 1/1000 dilution

All lanes:

H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 93 kDa

false

Western blot - Anti-Villin antibody (AB272876)
  • WB

Supplier Data

Western blot - Anti-Villin antibody (AB272876)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Villin antibody (ab272876) at 1/1000 dilution

All lanes:

Mouse kidney tissue at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human VIL1. The exact immunogen used to generate this antibody is proprietary information.

P09327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Helicobacter 26:e12849 PubMed34490965

2021

Upregulation of oncogene Activin A receptor type I by Helicobacter pylori infection promotes gastric intestinal metaplasia via regulating CDX2.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yan Chen,Yi Hu,Xin-Bo Xu,Yan-An Zhou,Nian-Shuang Li,Cong He,Chuan Xie,Nong-Hua Lu,Yin Zhu
View all publications
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Product promise

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