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AB97512

Anti-Villin antibody

4

(1 Review)

|

(10 Publications)

Rabbit Polyclonal Villin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Dog, Cat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 1-300.

View Alternative Names

VIL, VIL1, Villin-1

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Paraffin-embedded A549 xenograft tissue stained Villin using ab97512 at a 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB97512)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB97512)

Confocal immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Villin with ab97512 (green) at 1/500 dilution. Red : Alpha-tubulin filaments, alpha Tubulin antibody at 1/2000.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Paraffin-embedded rat kidney tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Paraffin-embedded mouse kidney tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Paraffin-embedded rat colon tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Paraffin-embedded mouse duodenum tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Paraffin-embedded mouse duodenum tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Immunohistochemical analysis of paraffin-embedded dog intestine tissue stained for VIL1 protein at cell membrane with ab97512 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Immunohistochemical analysis of paraffin-embedded cat colon tissue stained for VIL1 protein at cell membrane with ab97512 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

Immunohistochemical analysis of paraffin-embedded dog colon tissue stained for VIL1 protein at cell membrane with ab97512 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Cat, Dog

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P09327

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26118 PubMed40681631

2025

Nylon mesh chip promotes three-dimensional visualization of intestinal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Tan,Yao Li,Maolin Wang,Hengjun Huang,Jiayuan Li,Yangwen Ai,Jian Liu,Hui Li,Yinju Liu

iScience 27:109563 PubMed38623332

2024

Cultivation, cryopreservation, and transcriptomic studies of host-adapted and using enteroids.

Applications

Unspecified application

Species

Unspecified reactive species

Miner Deng,Tianyi Hou,Jie Zhang,Xinjie Mao,Fuxian Yang,Yanting Wei,Yongping Tang,Wanting Zeng,Wanyi Huang,Na Li,Lihua Xiao,Yaoyu Feng,Yaqiong Guo

International journal of molecular sciences 24: PubMed37958655

2023

A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid.

Applications

Unspecified application

Species

Unspecified reactive species

Yinju Liu,Jinlong Tan,Nianzhang Zhang,Wenhui Li,Baoquan Fu

International journal of molecular sciences 24: PubMed37686373

2023

A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Tan,Yinju Liu,Weike Li,Guohua Chen,Yongxiang Fang,Xiaobing He,Baoquan Fu,Zhizhong Jing

Transgenic research 32:305-319 PubMed37247123

2023

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Chuan Zheng,Hang Xue,Hong-Zhi Sun,Wen-Jing Yun,Zheng-Guo Cui

Cellular and molecular gastroenterology and hepatology 15:741-764 PubMed36521659

2022

Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn's Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Rizzo,Federica Rubbino,Sudharshan Elangovan,Giusy Sammarco,Sara Lovisa,Silvia Restelli,Samuel Elias Pineda Chavez,Luca Massimino,Luigi Lamparelli,Marianna Paulis,Annalisa Maroli,Giulia Roda,Mohammad Shalaby,Michele Carvello,Caterina Foppa,Sheona P Drummond,Paola Spaggiari,Federica Ungaro,Antonino Spinelli,Alberto Malesci,Alessandro Repici,Anthony J Day,Alessandro Armuzzi,Silvio Danese,Stefania Vetrano

Oxidative medicine and cellular longevity 2022:4877275 PubMed35308175

2022

Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hexin Wen,Xiaofeng Zhang,Qingqing Li,Ju Huang,Guangyong Liu,Jingyue Zhao,Yiran Liu,Li Shen,Yuyang Li,Kun Yang,Lugen Zuo,Jing Li,Jing Nian,Ping Xiang,Hao Zhao,Liang Yu,Mulin Liu,Zhijun Geng,Xue Song

ALTEX 37:255-264 PubMed31893489

2020

In vitro demonstration of intestinal absorption mechanisms of different sugars using 3D organotypic tissues in a fluidic device.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Marrella,Paolo Buratti,Jan Markus,Giuseppe Firpo,Mario Pesenti,Tim Landry,Seyoum Ayehunie,Silvia Scaglione,Helena Kandarova,Maurizio Aiello

The Biochemical journal 475:2057-2071 PubMed29871874

2018

Effect of the actin- and calcium-regulating activities of ITPKB on the metastatic potential of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Selina Bäder,Elina Glaubke,Saskia Grüb,Stefanie Muhs,Jasmin Wellbrock,Marcus Nalaskowski,Tobias Lange,Sabine Windhorst

Pharmaceutical research 35:72 PubMed29476278

2018

Human Primary Cell-Based Organotypic Microtissues for Modeling Small Intestinal Drug Absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Seyoum Ayehunie,Tim Landry,Zachary Stevens,Alex Armento,Patrick Hayden,Mitchell Klausner
View all publications
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