Anti-Villin antibody [EPR3491(3)] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [EPR3491(3)] - BSA and Azide free (AB248530)

This data was developed using ab133510, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Villin with ab133510 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [EPR3491(3)] - BSA and Azide free (AB248530)

This data was developed using ab133510, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling Villin with ab133510 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [EPR3491(3)] - BSA and Azide free (AB248530)

This data was developed using ab133510, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Villin with ab133510 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-Villin antibody [EPR3491(3)] - BSA and Azide free (AB248530)

This data was developed using ab133510, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Villin antibody [EPR3491(3)] (<a href='/en-us/products/primary-antibodies/villin-antibody-epr34913-ab133510'>ab133510</a>) at 1/10000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

HT-29 cell lysate at 10 µg

Lane 3:

Human small intestine tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 93 kDa

false

• WB

Lab

Western blot - Anti-Villin antibody [EPR3491(3)] - BSA and Azide free (AB248530)

This data was developed using the same antibody clone in a different buffer formulation (ab133510).

Western blot : Anti-VIL1 antibody [EPR3491(3)] (ab133510) staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab133510 was shown to bind specifically to VIL1. A band was observed at 93 kDa in wild-type A549 cell lysates with no signal observed at this size in VIL1 knockout cell line. To generate this image, wild-type and VIL1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Villin antibody [EPR3491(3)] (<a href='/en-us/products/primary-antibodies/villin-antibody-epr34913-ab133510'>ab133510</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lanes 2 and 4:

Empty cell lysate at 20 µg

Lane 3:

VIL1 knockout A549 cell lysate at 20 µg

Lane 5:

HepG2 cell lysate at 20 µg

Lane 6:

Jurkat Nuclear cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 93 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Villin antibody [EPR3491(3)] - BSA and Azide free (AB248530)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.