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AB317112

Anti-Villin antibody [HL1830]

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Rabbit Monoclonal Villin antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1.

View Alternative Names

VIL, VIL1, Villin-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [HL1830] (AB317112)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [HL1830] (AB317112)

ab317112 detects Villin protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse duodenum. Villin stained by ab317112 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Villin antibody [HL1830] (AB317112)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [HL1830] (AB317112)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317112 diluted at 1 : 4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Villin antibody [HL1830] (ab317112) at 1/4000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Villin antibody [HL1830] (AB317112)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [HL1830] (AB317112)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE and the membranes were blotted with ab317112 diluted at 1 : 4000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Villin antibody [HL1830] (ab317112) at 1/4000 dilution

All lanes:

HepG2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

false

Western blot - Anti-Villin antibody [HL1830] (AB317112)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [HL1830] (AB317112)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317112 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Villin antibody [HL1830] (ab317112) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

DDDDK-tagged Villin transfected (+) 293T whole cell extracts

Lane 3:

DDDDK-tagged Advillin transfected (+) 293T whole cell extracts

Lane 4:

DDDDK-tagged Gelsolin transfected (+) 293T whole cell extracts

Lane 5:

DDDDK-tagged Villin like transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1830

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human VIL1.

P09327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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