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AB268129

Anti-Villin antibody [rVIL1/1325]

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(1 Publication)

Mouse Monoclonal Villin antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 150-350.

View Alternative Names

VIL, VIL1, Villin-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] (AB268129)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] (AB268129)

Formalin-fixed paraffin-embedded human small intestinal carcinoma tissue stained for Villin using ab268129 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] (AB268129)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] (AB268129)

Formalin-fixed paraffin-embedded human small intestinal carcinoma tissue stained for Villin using ab268129 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Villin antibody [rVIL1/1325] (AB268129)
  • Protein Array

Unknown

Protein Array - Anti-Villin antibody [rVIL1/1325] (AB268129)

Protein Array containing more than 19000 full-length human proteins using ab268129.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

rVIL1/1325

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P09327

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 25:387 PubMed39478440

2024

The prognostic role of activation of the complement pathways in the progression of advanced IgA nephropathy to end-stage renal disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Shimin Jiang,Dingxin Di,Guming Zou,Hongmei Gao,Shunlai Shang,Wenge Li
View all publications

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